Binding of Radioactive Benzylpenicillin to Sporulating Bacillus Cultures: Chemistry and Fluctuations in Specific Binding Capacity

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Nov 1

PMID 4942758

Citations 10

Authors

P J Lawrence

M Rogolsky

V T Hanh

Affiliations

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Abstract

The chemistry of the binding of (14)C-benzylpenicillin to sporulating cultures of Bacillus megaterium and B. subtilis is similar to that in a 4-hr vegetative culture of Staphylococcus aureus. Unlabeled penicillins prevent the binding of (14)C-benzylpenicillin, but benzylpenicilloic acid and benzylpenilloic acid do not. Bound antibiotic can be removed from cells with neutral hydroxylamine at 25 C. Sporulating cultures display two intervals of enhanced binding, whereas binding to stationaryphase S. aureus cells remains constant. The first period of increased binding activity occurs during formation of the spore septum or cell wall primordium development, and the second coincides with cortex biosynthesis.

Citing Articles

Stability and synthesis of the penicillin-binding proteins during sporulation.

BUCHANAN C, Sowell M J Bacteriol. 1983; 156(2):545-51.

PMID: 6415034 PMC: 217866. DOI: 10.1128/jb.156.2.545-551.1983.

Changes in penicillin-binding proteins during sporulation of Bacillus subtilis.

Sowell M, BUCHANAN C J Bacteriol. 1983; 153(3):1331-7.

PMID: 6402492 PMC: 221781. DOI: 10.1128/jb.153.3.1331-1337.1983.

Binding of radioactive benzylpenicillin to asporogenous mutants of Bacillus subtilis during postexponential growth.

Rogolsky M, Lawrence P, Hanh V J Bacteriol. 1973; 114(1):220-7.

PMID: 4633343 PMC: 251758. DOI: 10.1128/jb.114.1.220-227.1973.

Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.

Blumberg P, Strominger J Bacteriol Rev. 1974; 38(3):291-335.

PMID: 4608953 PMC: 413858. DOI: 10.1128/br.38.3.291-335.1974.

Nature of the genetic determinant controlling exfoliative toxin production in Staphylococcus aureus.

Rogolsky M, Warren R, Wiley B, Nakamura H, Glasgow L J Bacteriol. 1974; 117(1):157-65.

PMID: 4271807 PMC: 246537. DOI: 10.1128/jb.117.1.157-165.1974.

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