Plasmid Segregation into Minicells is Associated with Membrane Attachment and Independent of Plasmid Replication

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1986 Jan 1

PMID 3510193

Citations 3

Authors

A Rashtchian

S W Brown

J Reichler

S B Levy

Affiliations

Soon will be listed here.

Abstract

The role of plasmid replication in the segregation of plasmids into Escherichia coli minicells was investigated with temperature-sensitive replication mutants derived from E. coli plasmids ColE1 and pSC101. For as long as six generations of growth, at permissive or nonpermissive temperatures (when greater than 80% of plasmid replication was inhibited), the same amount of previously 3H-labeled plasmid DNA segregated into minicells. Density gradient separations of wild-type and temperature-sensitive plasmid DNA from both replicons segregated into the minicells showed that about 20 to 25% was stably associated with the minicell membrane at both temperatures. Electron microscopy showed this DNA to consist of circular plasmid molecules attached to the minicell membrane. These combined findings suggest that segregation of plasmids into minicells and their association with the minicell membrane are interrelated and independent of plasmid replication.

Citing Articles

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Formation of supercoiling domains in plasmid pBR322.

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References

Meacock P, Cohen S . Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell. 1980; 20(2):529-42. DOI: 10.1016/0092-8674(80)90639-x. View

Gustafsson P, Wolf-Watz H, Lind L, Johansson K, Nordstrom K . Binding between the par region of plasmids R1 and pSC101 and the outer membrane fraction of the host bacteria. EMBO J. 1983; 2(1):27-32. PMC: 555081. DOI: 10.1002/j.1460-2075.1983.tb01375.x. View

Clewell D, Helinski D . Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969; 62(4):1159-66. PMC: 223628. DOI: 10.1073/pnas.62.4.1159. View

Collins J, Yanofsky S, Helinski D . Altered DNA-protein relaxation complex in a replication mutant of plasmid ColE1. Mol Gen Genet. 1978; 167(1):21-8. DOI: 10.1007/BF00270317. View

Sueoka N, Hammers J . Isolation of DNA-membrane complex in Bacillus subtilis. Proc Natl Acad Sci U S A. 1974; 71(12):4787-91. PMC: 433982. DOI: 10.1073/pnas.71.12.4787. View

Kline B, Miller J . Detection of nonintegrated plasmid deoxyribonucleic acid in the folded chromosome of Escherichia coli: physiochemical approach to studying the unit of segregation. J Bacteriol. 1975; 121(1):165-72. PMC: 285627. DOI: 10.1128/jb.121.1.165-172.1975. View

Hochmannova J, Nesvera J, Stokrova J, Perebityuk A . Intracellular location of NR1-plasmid DNA in Escherichia coli minicells. Folia Microbiol (Praha). 1981; 26(4):270-6. DOI: 10.1007/BF02927250. View

Nagai K, Hendrickson W, Balakrishnan R, Yamaki H, Boyd D, Schaechter M . Isolation of a replication origin complex from Escherichia coli. Proc Natl Acad Sci U S A. 1980; 77(1):262-6. PMC: 348249. DOI: 10.1073/pnas.77.1.262. View

Bedbrook J, Lehrach H, Ausubel F . Directive segregation in the basis of colE1 plasmid incompatibility. Nature. 1979; 281(5731):447-52. DOI: 10.1038/281447a0. View

10.

Levy S . Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells. J Bacteriol. 1971; 108(1):300-8. PMC: 247066. DOI: 10.1128/jb.108.1.300-308.1971. View

11.

Adler H, Fisher W, Cohen A, Hardigree A . MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967; 57(2):321-6. PMC: 335508. DOI: 10.1073/pnas.57.2.321. View

12.

Hendrickson W, Kusano T, Yamaki H, Balakrishnan R, King M, Murchie J . Binding of the origin of replication of Escherichia coli to the outer membrane. Cell. 1982; 30(3):915-23. DOI: 10.1016/0092-8674(82)90296-3. View

13.

Sparks Jr R, Helinski D . Association of cellular membrane of E. coli minicells with the origins/terminus region of replication of plasmid ColEl DNA. Nature. 1979; 277(5697):572-4. DOI: 10.1038/277572a0. View

14.

Levy S . The problems of drug-resistant pathogenic bacteria. Studies on R factors segregated into E. coli minicells. Ann N Y Acad Sci. 1971; 182:217-25. DOI: 10.1111/j.1749-6632.1971.tb30658.x. View

15.

FRAZER A, Curtiss 3rd R . Production, properties and utility of bacterial minicells. Curr Top Microbiol Immunol. 1975; 69:1-84. DOI: 10.1007/978-3-642-50112-8_1. View

16.

Firshein W, Strumph P, Benjamin P, Burnstein K, Kornacki J . Replication of a low-copy-number plasmid by a plasmid DNA-membrane complex extracted from minicells of Escherichia coli. J Bacteriol. 1982; 150(3):1234-43. PMC: 216345. DOI: 10.1128/jb.150.3.1234-1243.1982. View

17.

Hogan J, Kline B, Levy S . Regions on the F plasmid which affect plasmid maintenance and the ability to segregate into Escherichia coli minicells. Plasmid. 1982; 8(1):36-44. DOI: 10.1016/0147-619x(82)90039-7. View

18.

Scott J . Regulation of plasmid replication. Microbiol Rev. 1984; 48(1):1-23. PMC: 373000. DOI: 10.1128/mr.48.1.1-23.1984. View

19.

Winston S, Sueoka N . DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis. Proc Natl Acad Sci U S A. 1980; 77(5):2834-8. PMC: 349499. DOI: 10.1073/pnas.77.5.2834. View

20.

Wolf-Watz H, Norqvist A . Deoxyribonucleic acid and outer membrane: binding to outer membrane involves a specific protein. J Bacteriol. 1979; 140(1):43-9. PMC: 216777. DOI: 10.1128/jb.140.1.43-49.1979. View