Lymphoblastoid Transformation and Kinetics of Appearance of Viral Nuclear Antigen (EBNA) in Cord-blood Lymphocytes Infected by Epstein-Barr Virus (EBV)

Overview

Journal Int J Cancer

Specialty Oncology

Date 1975 Mar 15

PMID 49325

Citations 13

Authors

J Yata

C Desgranges

T Nakagawa

M C Favre

Affiliations

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Abstract

Human cord-blood lymphocytes were infected with B95.8 Epstein-Barr virus (EBV) before and after separation into B- and T-cell populations. Lymphoblastoid cells exhibiting B-cell characteristics appeared after 2 to 3 days of culture in the total population and in the separated B-cell subpopulation but not in the T-cell subpopulation. EBV nuclear antigen (EBNA) was detected concurrently with the appearance of lymphoblastoid cells. The proportion of EBNA-positive cells corresponded to that of lymphoblastoid cells, and reached 50% after 4 days. EBNA was present only in cells with B-cell markers. These observations indicate that only B-cells are susceptible to EBV infection, that the transformation occurs within a few days and that EBNA is a valid early marker for susceptibility to EBV transformation.

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