Cellular Differentiation of the Immune System of Mice. VI. Strain Differences in Class Differentiation and Other Properties of Marrow Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1970 Oct 1

PMID 4927657

Citations 8

Authors

G CUDKOWICZ

G M Shearer

T Ito

Affiliations

Soon will be listed here.

Abstract

Marrow cells and 5 x 10(7) thymocytes of unprimed (C57BL/6 x DBA/2)F(1), (C57BL/10 x WB)F(1) and (C3H x C57BL)F(1) donor mice were mixed in vitro and transplanted into X-irradiated syngeneic hosts. Upon injection of sheep erythrocytes, splenic plaque-forming cells (PFC) secreting IgM (direct PFC or IgG (indirect PFC) hemolytic antibody were enumerated at the time of peak responses. By grading the numbers of marrow cells, inocula were found that contained few immunocompetent cells reaching the recipient spleens, interacting with thymocytes or other accessory cells (or both), and generating PFC. The frequency of responses in BDF(1) mice conformed to Poisson statistics, indicating that immunocompetent marrow cells participated in a single-hit interaction limiting PFC responses. The marrow cells assayed were not restricted for the antibody class (IgM versus IgG) to be secreted by mature PFC. Unrestricted marrow cells could have been either the precursors of PFC or accessory cells. Different results were obtained in BWF(1) and C3BF(1) mice. The frequency of responses in relation to the number of marrow cells grafted did not follow Poisson statistics, and the limiting cells were restricted for antibody class. Presumably, immunocompetent cells of these strains were more heterogeneous than those of BDF(1) mice and participated in a multiplicity of cell-to-cell interactions. The strain differences reflected inherent properties of marrow cells and not influences of the environment in which PFC were produced. The results confirmed for bone marrow the heterogeneity of immunocompetent cells reported by others for spleen, and suggested that genetic factors such as "immune response" genes regulate cellular differentiation also for functions other than those related to antibody specificity.

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References

Shearer G, CUDKOWICZ G, Priore R . Cellular differentiation of the immune system of mice. IV. Lack of class differentiation in thymic antigen-reactive cells. J Exp Med. 1969; 130(3):467-80. PMC: 2138712. DOI: 10.1084/jem.130.3.467. View

Mosier D, Coppleson L . A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro. Proc Natl Acad Sci U S A. 1968; 61(2):542-7. PMC: 225193. DOI: 10.1073/pnas.61.2.542. View

Shearer G, CUDKOWICZ G, Priore R . Cellular differentiation of the immune system of mice. II. Frequency of unipotent splenic antigen-sensitive units after immunization with sheep erythrocytes. J Exp Med. 1969; 129(1):185-99. PMC: 2138596. DOI: 10.1084/jem.129.1.185. View

Sell S . Studies on rabbit lymphocytes in vitro. V. The induction of blast transformation with sheep antisera to rabbit IgG subunits. J Exp Med. 1967; 125(2):289-301. PMC: 2138360. DOI: 10.1084/jem.125.2.289. View

Takahashi M, Takagi N, Yagi Y, Moore G, Pressman D . Immunoglobulin production in cloned sublines of a human lymphocytoid cell line. J Immunol. 1969; 102(6):1388-93. View

Sell S, Lowe J, Gell P . Studies on rabbit lymphocytes in vitro. XI. Superaddition of anti-allotypic lymphocyte transformation: evidence for multipotent lymphoid cells. J Immunol. 1970; 104(1):103-13. View

Davies A . The thymus and the cellular basis of immunity. Transplant Rev. 1969; 1:43-91. DOI: 10.1111/j.1600-065x.1969.tb00136.x. View

Shearer G, CUDKOWICZ G . Distinct events in the immune response elicited by transferred marrow and thymus cells. I. Antigen requirements and priferation of thymic antigen-reactive cells. J Exp Med. 1969; 130(6):1243-61. PMC: 2138694. DOI: 10.1084/jem.130.6.1243. View

McDevitt H, TYAN M . Genetic control of the antibody response in inbred mice. Transfer of response by spleen cells and linkage to the major histocompatibility (H-2) locus. J Exp Med. 1968; 128(1):1-11. PMC: 2138514. View

10.

Friedman H . DISTRIBUTION OF ANTIBODY PLAQUE FORMING CELLS IN VARIOUS TISSUES OF SEVERAL STRAINS OF MICE INJECTED WITH SHEEP ERYTHROCYTES. Proc Soc Exp Biol Med. 1964; 117:526-30. DOI: 10.3181/00379727-117-29628. View

11.

Groves D, Lever W, Makinodan T . A model for the interaction of cell types in the generation of hemolytic plaque-forming cells. J Immunol. 1970; 104(1):148-65. View

12.

Playfair J . Strain differences in the immune response of mice. I. The neonatal response to sheep red cells. Immunology. 1968; 15(1):35-50. PMC: 1409424. View

13.

Shearer G, CUDKOWICZ G . Cellular differentiation of the immune system of mice. 3. Separate antigen-sensitive units for different types of anti-sheep immunocytes formed by marrow-thymus cell mixtures. J Exp Med. 1969; 129(5):935-51. PMC: 2138636. DOI: 10.1084/jem.129.5.935. View

14.

Playfair J . Strain differences in the immune response of mice. II. Responses by neonatal cells in irradiated adult hosts. Immunology. 1968; 15(6):815-26. PMC: 1409502. View

15.

Takahashi M, TANIGAKI N, Yagi Y, Moore G, Pressman D . Presence of two different immunoglobulin heavy chains in individual cells of established human hematopoietic cell lines. J Immunol. 1968; 100(6):1176-83. View

16.

Mosier D, Fitch F, Rowley D, Davies A . Cellular deficit in thymectomized mice. Nature. 1970; 225(5229):276-7. DOI: 10.1038/225276a0. View

17.

MORTON J, Siegel B . Response of NZB mice to foreign antigen and development of autoimmune disease. J Reticuloendothel Soc. 1969; 6(1):78-93. View

18.

CUDKOWICZ G, Shearer G, Priore R . Cellular differentiation of the immune system of mice. V. Class differentiation in marrow precursors of plaque-forming cells. J Exp Med. 1969; 130(3):481-91. PMC: 2138708. DOI: 10.1084/jem.130.3.481. View

19.

Barth R, Carroll G . Immunosuppressive effects of antilymphocyte serum in complement-deficient mice: evidence that immune cytolysis is not essential for ALS activity. J Immunol. 1970; 104(2):522-4. View

20.

Mozes E, Shearer G, Sela M . Cellular basis of the genetic control of immune responses to synthetic polypeptides. I. Differences in frequency of splenic precursor cells specific for a synthetic polypeptide derived from multichain polyproline ((T,G)-Pro--L) in high and low.... J Exp Med. 1970; 132(4):613-22. PMC: 2138860. DOI: 10.1084/jem.132.4.613. View