Translational Repression in the Arginine System of Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1970 Dec 1

PMID 4923118

Citations 19

Authors

W L MCLELLAN

H J Vogel

Affiliations

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Abstract

Translation of bacterial mRNA, divorced from transcription, has been obtained for enzymes of arginine synthesis; evidence has been acquired for repression by arginine at the level of translation. mRNAs for acetylornithinase and ornithine transcarbamylase were accumulated by arginine starvation of argR(+) and argR(-) arginine auxotrophs derived from Escherichia coli K12. Further transcription was inhibited with rifampicin or miracil D, and enzyme formation was measured in the presence of either an excess of, or a restricted supply of, arginine. For the argR(+) strain 961, little mRNA was found without starvation; for the argR(-) strain 977, a considerable amount of mRNA was demonstrated even without starvation. There was relatively little translation for the argR(+) strain, but not for the argR(-) strain, in the presence of excess arginine, apparently due to an accelerated degradation of mRNA in the argR(+) strain under repressive conditions.

Citing Articles

Involvement of threonine deaminase in multivalent repression of the isoleucine-valine pathway in Saccharomyces cerevisiae.

Bollon A, Magee P Proc Natl Acad Sci U S A. 1971; 68(9):2169-72.

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On the control of the induction of -galactosidase in synchronous cultures of Escherichia coli.

Goldberg R, Chargaff E Proc Natl Acad Sci U S A. 1971; 68(8):1702-6.

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Isoleucine and valine metabolism in Escherichia coli K-12: detection and measurement of ilv-specific messenger ribonucleic acid.

Vonder Haar R, Umbarger H J Bacteriol. 1974; 120(2):687-96.

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Participation of branched-chain amino acid analogues in multivalent repression.

Wasmuth J, Umbarger H J Bacteriol. 1973; 116(2):562-70.

PMID: 4583240 PMC: 285418. DOI: 10.1128/jb.116.2.562-570.1973.

Transient regulation of enzyme synthesis in Escherichia coli.

Boy E, Theze J, PATTE J Mol Gen Genet. 1973; 121(1):77-81.

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