New Assay Procedure for Separation of Mycoplasmas from Virus Pools and Tissue Culture Systems

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1967 Dec 1

PMID 4912246

Citations 14

Authors

I ZGORNIAK-NOWOSIELSKA

W D Sedwick

K Hummeler

H KOPROWSKI

Affiliations

Soon will be listed here.

Abstract

Presence of mycoplasma organisms in tissue culture systems and virus pools was detected by titration of the contaminated material on agarose-suspended BHK21/13S cells. The use of this method permitted isolation of mycoplasmas which could not be detected by standard assay methods. Mycoplasma colonies at concentrations ranging from 10(4) to 10(6) colony-forming units/ml in agarose-BHK21/13S media could be distinguished from virus plaques, and the two populations of microorganisms could be easily disassociated either by electron microscopy or by biological methods. All isolated mycoplasmas were identified in growth inhibition tests as belonging to the GDL group. The growth inhibition test on agarose-BHK21/13S cell suspension plates could also be applied directly to those strains which could not be isolated by standard assay procedures.

Citing Articles

Altered nucleic acid metabolism in human cell cultures infected with mycoplasma.

Levine E, Thomas L, McGregor D, Hayflick L, Eagle H Proc Natl Acad Sci U S A. 1968; 60(2):583-9.

PMID: 5248816 PMC: 225087. DOI: 10.1073/pnas.60.2.583.

Elimination of mycoplasma from various cell cultures.

Vogelzang A Antonie Van Leeuwenhoek. 1969; 35(4):393-408.

PMID: 4989532 DOI: 10.1007/BF02219158.

Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions.

Sedwick W, WIKTOR T J Virol. 1967; 1(6):1224-6.

PMID: 4987176 PMC: 375413. DOI: 10.1128/JVI.1.6.1224-1226.1967.

Plaque assay for Rickettsia rickettsii.

Weinberg E, Stakebake J, Gerone P J Bacteriol. 1969; 98(2):398-402.

PMID: 4977475 PMC: 284828. DOI: 10.1128/jb.98.2.398-402.1969.

Purification of rabies virus grown in tissue culture.

Sokol F, Kuwert E, WIKTOR T, Hummeler K, KOPROWSKI H J Virol. 1968; 2(8):836-49.

PMID: 4973571 PMC: 375698. DOI: 10.1128/JVI.2.8.836-849.1968.

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