Polarity in Gene AraB of the L-Arabinose Operon in Escherichia Coli B/r

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1969 Nov 1

PMID 4901356

Citations 6

Authors

D E Sheppard

D A Walker

Affiliations

Soon will be listed here.

Abstract

A series of mutations are described which map in the araB gene of the l-arabinose operon and exert a polar effect on gene araA, the structural gene for the l-arabinose isomerase. Ten of the 20 araB point mutants examined exhibited absolute polarity and may represent insertions of genetic material into the araB gene. The remaining 10 point mutants exhibit strong polarity (less than 10% of the normal wild-type inducible level of isomerase) and may represent a class of externally suppressible polar mutations other than amber or ochre. Seven of the 12 araB deletion mutants examined, or 58%, exhibit polarity, suggesting that a shift in the reading frame has been generated in the polycistronic message for the l-arabinose operon. The remaining, presumably in-phase, deletion mutants exhibit hyperinducible levels of isomerase, an effect that is eliminated when an araB(+) gene is introduced in the trans position. The hyperinducibility effect is discussed in terms of a model for self-catabolite repression, originally proposed by Katz and Englesberg.

Citing Articles

D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC.

Beverin S, Sheppard D, Park S J Bacteriol. 1971; 107(1):79-86.

PMID: 4935332 PMC: 246888. DOI: 10.1128/jb.107.1.79-86.1971.

Characterization of strong polar mutations in a region immediately adjacent to the L-arabinose operator in Escherichia coli B-r.

Eleuterio M, Griffin B, Sheppard D J Bacteriol. 1972; 111(2):383-91.

PMID: 4559731 PMC: 251294. DOI: 10.1128/jb.111.2.383-391.1972.

Mutations in the L-arabinose operon of Escherichia coli B-r that result in hypersensitivity to catabolite repression.

Gendron R, Sheppard D J Bacteriol. 1974; 117(2):417-21.

PMID: 4359644 PMC: 285528. DOI: 10.1128/jb.117.2.417-421.1974.

Hyperinducibility as a result of mutation in structural genes and self-catabolite repression in the ara operon.

Katz L, ENGLESBERG E J Bacteriol. 1971; 107(1):34-52.

PMID: 4327512 PMC: 246883. DOI: 10.1128/jb.107.1.34-52.1971.

The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r.

Bass R, Heffernan L, Sweadner K, ENGLESBERG E Arch Microbiol. 1976; 110(1):135-43.

PMID: 189718 DOI: 10.1007/BF00416978.

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