The Site for Catabolite Deactivation in the L-arabinose BAD Operon in Escherichia Coli B/r

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1976 Oct 11

PMID 189718

Citations 10

Authors

R Bass

L Heffernan

K Sweadner

E ENGLESBERG

Affiliations

Soon will be listed here.

Abstract

A series of deletions beginning in the leu operon and continuing into the araC gene and also into the ara controlling site region were analyzed in reciprocal merodiploids, e.g., F' A2Cc67/B24delta719, F' B24delta719/A2Cc67, for their effects on catabolite deactivation (CD). The results of these experiments are consistent with placing the catabolite gene activator-cyclic AMP sensitive site in the controlling site region between araB and araO. With a deletion mutant, delta1109, that places araBAD under leu control when transcription begins at leuP, the araBAD operon is immune to CD even though araCGA, araP and araI are intact and functional. To focus attention on the fine structure and related functions of this region we propose that the three proteins that function therein have separate sites of action: araI (initiator-site for activator), araP (promoter-site for RNA polymerase) and ara(CGA) (catabolite gene activator-site for CGA-cAMP). None of the eighteen initiator constitutive mutants (Ic) tested have any significant effect on catabolite derepression or on the maximal level of expression of the operon supporting the view that the araI site may be distinct from araP and ARA(CGA). A series of constitutive mutants in the araC gene (Cc) also have no pronounced effect on catabolite deactivation.

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References

Patrick J, Lee N, Barnes N, ENGLESBERG E . Coordination of enzyme synthesis in the L-arabinose operon in Escherichia coli. I. The effect of manganous ion on the synthesis of L-arabinose isomerase. J Biol Chem. 1971; 246(16):5102-6. View

GREENBLATT J, Schleif R . Arabinose C protein: regulation of the arabinose operon in vitro. Nat New Biol. 1971; 233(40):166-70. DOI: 10.1038/newbio233166a0. View

Sheppard D, Walker D . Polarity in gene araB of the l-Arabinose operon in Escherichia coli B/r. J Bacteriol. 1969; 100(2):715-23. PMC: 250149. DOI: 10.1128/jb.100.2.715-723.1969. View

Wilcox G, Clemetson K, Santi D, ENGLESBERG E . Purification of the araC protein. Proc Natl Acad Sci U S A. 1971; 68(9):2145-8. PMC: 389371. DOI: 10.1073/pnas.68.9.2145. View

Sheppard D, ENGLESBERG E . Positive control in the L-arabinose gene-enzyme complex of Escherichia coli B/r exhibited with stable merodiploids. Cold Spring Harb Symp Quant Biol. 1966; 31:345-7. DOI: 10.1101/sqb.1966.031.01.044. View

Wilcox G, Singer J, Heffernan L . Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r. J Bacteriol. 1971; 108(1):1-4. PMC: 247024. DOI: 10.1128/jb.108.1.1-4.1971. View

Heffernan L, Bass R, ENGLESBERG E . Mutations affecting catabolite repression of the L-arabinose regulon in Escherichia coli B/r. J Bacteriol. 1976; 126(3):1119-31. PMC: 233134. DOI: 10.1128/jb.126.3.1119-1131.1976. View

Perlman R, de Crombrugghe B, Pastan I . Cyclic AMP regulates catabolite and transient repression in E. coli. Nature. 1969; 223(5208):810-2. DOI: 10.1038/223810a0. View

Ullmann A, MONOD J . Cyclic AMP as an antagonist of catabolite repression in Escherichia coli. FEBS Lett. 1968; 2(1):57-60. DOI: 10.1016/0014-5793(68)80100-0. View

10.

De Crombrugghe E, Chen B, Anderson W, GOTTESMAN M, Perlman R, Pastan I . Role of cyclic adenosine 3',5'-monophosphate and the cyclic adenosine 3',5'-monophosphate receptor protein in the initiation of lac transcription. J Biol Chem. 1971; 246(23):7343-8. View

11.

Wilcox G, Clemetson K, Cleary P, ENGLESBERG E . Interaction of the regulatory gene product with the operator site in the L-arabinose operon of Escherichia coli. J Mol Biol. 1974; 85(4):589-602. DOI: 10.1016/0022-2836(74)90317-9. View

12.

Emmer M, deCrombrugghe B, Pastan I, Perlman R . Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes. Proc Natl Acad Sci U S A. 1970; 66(2):480-7. PMC: 283070. DOI: 10.1073/pnas.66.2.480. View

13.

Perlman R, Pastan I . Regulation of beta-galactosidase synthesis in Escherichia coli by cyclic adenosine 3',5'-monophosphate. J Biol Chem. 1968; 243(20):5420-7. View

14.

Lee N, Wilcox G, Gielow W, Arnold J, Cleary P, ENGLESBERG E . In vitro activation of the transcription of araBAD operon by araC activator. Proc Natl Acad Sci U S A. 1974; 71(3):634-8. PMC: 388066. DOI: 10.1073/pnas.71.3.634. View

15.

Power J, Irr J . Regulatory gene control of transcription of the L-arabinose operon in Escherichia coli. J Biol Chem. 1973; 248(22):7806-10. View

16.

Zubay G, Schwartz D, Beckwith J . Mechanism of activation of catabolite-sensitive genes: a positive control system. Proc Natl Acad Sci U S A. 1970; 66(1):104-10. PMC: 286094. DOI: 10.1073/pnas.66.1.104. View

17.

Wilcox G, Meuris P, Bass R, ENGLESBERG E . Regulation of the L-arabinose operon BAD in vitro. J Biol Chem. 1974; 249(9):2946-52. View

18.

ENGLESBERG E, Irr J, Power J, Lee N . Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol. 1965; 90(4):946-57. PMC: 315760. DOI: 10.1128/jb.90.4.946-957.1965. View

19.

Katz L, ENGLESBERG E . Hyperinducibility as a result of mutation in structural genes and self-catabolite repression in the ara operon. J Bacteriol. 1971; 107(1):34-52. PMC: 246883. DOI: 10.1128/jb.107.1.34-52.1971. View

20.

Heffernan L, Wilcox G . Effect of araC gene product on catabolite repression in the L-arabinose regulon. J Bacteriol. 1976; 126(3):1132-5. PMC: 233135. DOI: 10.1128/jb.126.3.1132-1135.1976. View