Properties of a Cell Fraction That Repairs Damage to the Cell Division Mechanism of Escherichia Coli
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A factor in bacterial cell extracts which induces cell division in filaments of Escherichia coli produced by irradiation was found to be associated with a heat-labile particulate fraction which sediments at about 100S. Frozen or lyophilized samples of the cell extracts were stable for considerable periods of time. Fractions purified by centrifugation contained 2% of the total protein of the extract but no measurable ribonucleic acid or deoxyribonucleic acid. The extracts were inactivated by incubation with phospholipase A, lipases, and detergents, but not by incubation with selected nucleases and proteases.
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