Quantitative Analysis of Cholesterol in 5 to 20 Microliter of Plasma

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 1974 May 1

PMID 4827918

Citations 43

Authors

T T Ishikawa

J MACGEE

J A Morrison

C J Glueck

Affiliations

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Abstract

A gas-liquid chromatographic micromethod for quantitation of cholesterol in 20 micro l of plasma was developed using 5alpha-cholestane as an internal standard, saponification with tetramethylammonium hydroxide-isopropanol, and extraction with tetrachloroethylene-methyl butyrate. Cholesterol levels in plasma samples were calculated by comparing cholesterol-cholestane peak height ratios with those of preassayed reference plasma. Over a plasma cholesterol range of 44 to 468 mg/100 ml, the gas-liquid chromatographic micromethod and the automated ferric chloride colorimetric method gave nearly identical results (r = 0.99) in duplicate aliquots of 131 plasma samples.

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