Synthesis of Cephalosporin C from Sulfate by Mutants of Cephalosporium Acremonium

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1973 Oct 1

PMID 4799669

Authors

H F NISS

C H Nash 3rd

Affiliations

Soon will be listed here.

Abstract

The innate ability of Cephalosporium acremonium to use methionine preferentially over sulfate for synthesis of cephalosporin C can be influenced through mutation. Mutants of C. acremonium with altered capacity to utilize sulfate for synthesis of antibiotic were isolated and partially characterized with respect to the uptake of sulfate and the regulation of arylsulfatase.

References

Marzluf G, Metzenberg R . Positive control by the cys-3 locus in regulation of sulfur metabolism in Neurospora. J Mol Biol. 1968; 33(2):423-37. DOI: 10.1016/0022-2836(68)90199-x. View

Caltrider P, NISS H . Role of methionine in cephalosporin synthesis. Appl Microbiol. 1966; 14(5):746-53. PMC: 1058408. DOI: 10.1128/am.14.5.746-753.1966. View

Marzluf G . Genetic and metabolic controls for sulfate metabolism in Neurospora crassa: isolation and study of chromate-resistant and sulfate transport-negative mutants. J Bacteriol. 1970; 102(3):716-21. PMC: 247617. DOI: 10.1128/jb.102.3.716-721.1970. View

Segel I, Johnson M . INTERMEDIATES IN INORGANIC SULFATE UTILIZATION BY PENICILLIUM CHRYSOGENUM. Arch Biochem Biophys. 1963; 103:216-26. DOI: 10.1016/0003-9861(63)90398-9. View

Lemke P . A century of compounds and their effect on fungi. Mycopathol Mycol Appl. 1969; 38(1):49-59. DOI: 10.1007/BF02051675. View

MONDOVI B, SCIOSCIA-SANTORO A, CAVALLINID . Further evidence on the identity of cystathionase and cysteine desulfhydrase. Arch Biochem Biophys. 1963; 101:363-4. DOI: 10.1016/s0003-9861(63)80025-9. View

Ott J, Godzeski C, Pavey D, Farran J, Horton D . Biosynthesis of Cephalosporin C: I. Factors Affecting the Fermentation. Appl Microbiol. 1962; 10(6):515-23. PMC: 1057906. DOI: 10.1128/am.10.6.515-523.1962. View

DEMAIN A, Newkirk J . Biosynthesis of cephalosporin C. Appl Microbiol. 1962; 10:321-5. PMC: 1057867. DOI: 10.1128/am.10.4.321-325.1962. View

Yamamoto L, Segel I . The inorganic sulfate transport system of Penicillium chrysogenum. Arch Biochem Biophys. 1966; 114(3):523-38. DOI: 10.1016/0003-9861(66)90376-6. View

10.

DENNEN D, Carver D . Sulfatase regulation and antibiotic synthesis in Cephalosporium acremonium. Can J Microbiol. 1969; 15(2):175-81. DOI: 10.1139/m69-029. View

11.

Stevens C, Vohra P, Inamine E, ROHOLT Jr O . Utilization of sulfur compounds for the biosynthesis of penicillins. J Biol Chem. 1953; 205(2):1001-6. View

12.

Flavin M, Slaughter C . The derepression and function of enzymes of reverse trans-sulfuration in Neurospora. Biochim Biophys Acta. 1967; 132(2):406-11. DOI: 10.1016/0005-2744(67)90159-3. View

13.

Segel I, Johnson M . Accumulation of intracellular inorganic sulfate by Penicillium chrysogenum. J Bacteriol. 1961; 81:91-8. PMC: 278959. DOI: 10.1128/jb.81.1.91-98.1961. View

14.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

15.

DELAVIER-KLUTCHKO C, Flavin M . ENZYMATIC SYNTHESIS AND CLEAVAGE OF CYSTATHIONINE IN FUNGI AND BACTERIA. J Biol Chem. 1965; 240:2537-49. View

16.

Metzenberg R, PARSON J . Altered repression of some enzymes of sulfur utilization in a temperature-conditional lethal mutant of Neurospora. Proc Natl Acad Sci U S A. 1966; 55(3):629-35. PMC: 224198. DOI: 10.1073/pnas.55.3.629. View

17.

NEWTON G, ABRAHAM E . Cephalosporin C, a new antibiotic containing sulphur and D-alpha-aminoadipic acid. Nature. 1955; 175(4456):548. DOI: 10.1038/175548a0. View