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Cephalosporinase Activity in Bacteroides Fragilis

Overview
Journal Antimicrob Agents Chemother
Publisher American Society for Microbiology
Specialty Pharmacology
Date 1973 Mar 1
PMID 4790596
Citations 20
Authors
V E Del Bene
W E Farrar Jr
Affiliations
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Abstract

Cephalosporinase activity was demonstrated in all of 10 strains of Bacteroides fragilis investigated. Low rates of hydrolysis of cephalosporins (0.25 to 3.5 mumol of cephaloridine per h per 10(9) cells) were found, but no activity against penicillin substrates was detected. In two strains the cephalosporinase activity was increased 40-and 80-fold by growing cells in the presence of penicillin. No permeability barrier for these antibiotics was demonstrated. In most cases the substrate profile showed decreasing activity in the following order: cephaloridine > cephalothin > cephaloglycin > cephalexin. The cephalosporinase from these organisms differed from that found in facultative and aerobic gram-negative bacilli in that it was inhibited by both cloxacillin and p-chloromercuribenzoate. Among nine strains of Bacteroides fragilis subspecies fragilis, correlation was found between in vitro resistance to cephaloridine and amount of beta-lactamase activity in sonically disrupted cells.

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