Increased Myocardial Catalase in Rats Fed Ethanol

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1979 Aug 1

PMID 474705

Citations 13

Authors

H D Fahimi

M Kino

L Hicks

K A Thorp

W H Abelman

Affiliations

Soon will be listed here.

Abstract

The effects of chronic intake of dietary ethanol upon catalase, an enzyme capable of metabolizing ethanol, as well as upon myocardial morphology and hemodynamics, were studied in the rat. Ethanol, comprising 36% of dietary calories, administered to rats for 5 weeks, was associated with increased myocardial catalase of 45.9 +/- 3.7 IU/mg protein, compared to 21.0 +/- 1.8 IU/mg protein in pair-fed controls. The enzyme activity remained significantly elevated after 18 weeks of ethanol. Hepatic catalase did not differ in these groups. Parallel cytochemical studies confirmed the increase in myocardial catalase by demonstrating an increase in peroxisomes. Gross and light-microscopic examinations revealed no abnormalities at either 5 or 18 weeks. Remarkably few ultrastructural abnormalities were seen in this material fixed by vascular perfusion. Hemodynamic studies after 5 weeks of ethanol revealed decreased left ventricle systolic pressure and decreased mean arterial pressure but no change in ventricular filling pressure. The possibility of catalase playing a metabolic and potentially protective role in rat myocardium chronically exposed to ethanol is discussed.

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References

HIBBS R, Ferrans V, Black W, WEILBAECHER D, BURCH G . ALCOHOLIC CARDIOMYOPATHY; AN ELECTRON MICROSCOPIC STUDY. Am Heart J. 1965; 69:766-79. DOI: 10.1016/0002-8703(65)90450-3. View

Appleman D, Heim W, Pyfrom H . Effects of 3-amino-1, 2, 4-triazole (AT) on catalase and other compounds. Am J Physiol. 1956; 186(1):19-23. DOI: 10.1152/ajplegacy.1956.186.1.19. View

Lieber C, Jones D, DECARLI L . EFFECTS OF PROLONGED ETHANOL INTAKE: PRODUCTION OF FATTY LIVER DESPITE ADEQUATE DIETS. J Clin Invest. 1965; 44:1009-21. PMC: 292581. DOI: 10.1172/JCI105200. View

GIMENO A, GIMENO M, Webb J . Effects of ethanol on cellular membrane potentials and contractility of isolated rat atrium. Am J Physiol. 1962; 203:194-6. DOI: 10.1152/ajplegacy.1962.203.1.194. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Alexander C, Sekhri K, Nagasawa H . Alcoholic cardiomyopathy in mice. Electron microscopic observations. J Mol Cell Cardiol. 1977; 9(3):247-54. DOI: 10.1016/0022-2828(77)90033-5. View

Alexander C, Forsyth G, Nagasawa H, Kohlhoff J . Alcoholic cardiomyopathy in mice. Myocardial glycogen, lipids and certain enzymes. J Mol Cell Cardiol. 1977; 9(3):235-45. DOI: 10.1016/0022-2828(77)90032-3. View

Hirota Y, BING O, Abelmann W . Effect of ethanol on contraction and relaxation of isolated rat ventricular muscle. J Mol Cell Cardiol. 1976; 08(9):727-32. DOI: 10.1016/0022-2828(76)90014-6. View

Hicks L, Fahimi H . Peroxisomes (microbodies) in the myocardium of rodents and primates. A comparative Ultrastructural cytochemical study. Cell Tissue Res. 1977; 175(4):467-81. DOI: 10.1007/BF00222413. View

10.

Stauber W, BIRD J, Schottelius B . Catalase: an enzymatic indicator of the degree of muscle wasting. Exp Neurol. 1977; 55(2):381-9. DOI: 10.1016/0014-4886(77)90008-5. View

11.

BURCH G, Colcolough H, Harb J, TSUI C . The effect of ingestion of ethyl alcohol, wine and beer on the myocardium of mice. Am J Cardiol. 1971; 27(5):522-8. DOI: 10.1016/0002-9149(71)90415-2. View

12.

Pachinger O, Tillmanns H, Mao J, Fauvel J, BING R . The effect of prolonged administration of ethanol on cardiac metabolism and performance in the dog. J Clin Invest. 1973; 52(11):2690-6. PMC: 302535. DOI: 10.1172/JCI107463. View

13.

Regan T, Khan M, Ettinger P, Haider B, LYONS M, OLDEWURTEL H . Myocardial function and lipid metabolism in the chronic alcoholic animal. J Clin Invest. 1974; 54(3):740-52. PMC: 301608. DOI: 10.1172/JCI107812. View

14.

SCHREIBER S, ORATZ M, Rothschild M, Reff F, Evans C . Alcoholic cardiomyopathy. II. The inhibition of cardiac microsomal protein synthesis by acetaldehyde. J Mol Cell Cardiol. 1974; 6(2):207-13. DOI: 10.1016/0022-2828(74)90023-6. View

15.

Thurman R, Scholz R . The role of hydrogen peroxide and catalase in hepatic microsomal ethanol oxidation. Drug Metab Dispos. 1973; 1(1):441-8. View

16.

RASKIN N, Sokoloff L . Enzymes catalysing ethanol metabolism in neural and somatic tissues of the rat. J Neurochem. 1972; 19(2):273-82. DOI: 10.1111/j.1471-4159.1972.tb01337.x. View

17.

SCHREIBER S, Briden K, ORATZ M, Rothschild M . Ethanol, acetaldehyde, and myocardial protein synthesis. J Clin Invest. 1972; 51(11):2820-6. PMC: 292430. DOI: 10.1172/JCI107104. View

18.

Lochner A, Cowley R, Brink A . Effect of ethanol on metabolism and function of perfused rat heart. Am Heart J. 1969; 78(6):770-80. DOI: 10.1016/0002-8703(69)90443-8. View

19.

Hall J, Rowlands Jr D . Cardiotoxicity of alcohol. An electron microscopic study in the rat. Am J Pathol. 1970; 60(2):153-64. PMC: 2032899. View

20.

Brin M, Dibble M, Peel A, McMullen E, BOURQUIN A, Chen N . Some preliminary findings on the nutritional status of the aged in Onondaga County, New York. Am J Clin Nutr. 1965; 17(4):240-58. DOI: 10.1093/ajcn/17.4.240. View