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New Colorimetric Method for the Quantitative Estimation of Phospholipids Without Acid Digestion

Overview
Journal J Lipid Res
Publisher Elsevier
Specialty Biochemistry
Date 1973 Nov 1
PMID 4742564
Citations 68
Authors
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Abstract

A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res., 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1-10 micro g of phospholipid phosphorus.

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