Activation of Brain Hexokinase by Magnesium Ions and by Magnesium Ion--adenosine Triphosphate Complex

Overview

Journal Biochem J

Specialty Biochemistry

Date 1972 Nov 1

PMID 4655453

Citations 4

Authors

D L Purich

H J FROMM

Affiliations

Soon will be listed here.

Abstract

1. An alternative explanation for the kinetic data obtained by Bachelard (1971) for the brain hexokinase reaction is presented. 2. Apparently sigmoidal saturation curves for MgATP(2-) based upon Bachelard's (1971) studies can be corrected to hyperbolic curves by use of a stability constant for MgATP(2-) complex formation. 3. A number of other effects related to the concentration-dependent stability of the MgATP(2-) complex and to the presence of the inhibitory free uncomplexed ATP(4-) concentration are also explained in terms of a non-allosteric role for either Mg(2+) or MgATP(2-) fully consistent with a number of previous reports on this enzyme. 4. A brief discussion of the validity of Hill plots in studies of multisubstrate co-operative enzymes is presented. 5. A simple model is presented that demonstrates how enzymes obeying Michaelis-Menten kinetics can demonstrate sigmoidal velocity responses if the true substrate of the reaction is the metal-substrate complex.

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Microcalorimetric study of magnesium-adenosine triphosphate ternary complex.

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Difference in kinetic properties between hexokinase type I isoenzymes from various rat tissues with reference to the effect of a thiol inhibitor.

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Differences in catalytic properties between cerebral cytoplasmic and mitochondrial hexokinases.

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References

Keech B, Barritt G . Allosteric activation of sheep kidney pyruvate carboxylase by the magnesium ion (Mg2+) and the magnesium adenosine triphosphate ion (MgATP2-). J Biol Chem. 1967; 242(9):1983-7. View

Copley M, FROMM H . Kinetic studies of the brain hexokinase reaction. A reinvestigation with the solubilized bovine enzyme. Biochemistry. 1967; 6(11):3503-9. DOI: 10.1021/bi00863a023. View

KOSOW D, Rose I . Activators of yeast hexokinase. J Biol Chem. 1971; 246(8):2618-25. View

Atkinson D, HATHAWAY J, Smith E . KINETICS OF REGULATORY ENZYMES. KINETIC ORDER OF THE YEAST DIPHOSPHOPYRIDINE NUCLEOTIDE ISOCITRATE DEHYDROGENASE REACTION AND A MODEL FOR THE REACTION. J Biol Chem. 1965; 240:2682-90. View

Rudolph F, FROMM H . Initial rate studies of adenylosuccinate synthetase with product and competitive inhibitors. J Biol Chem. 1969; 244(14):3832-9. View

Peck Jr E, Ray Jr W . Metal complexes of phosphoglucomutase in vivo. Alterations induced by insulin. J Biol Chem. 1971; 246(4):1160-7. View

OSullivan W, Perrin D . THE STABILITY CONSTANTS OF METAL-ADENINE NUCLEOTIDE COMPLEXES. Biochemistry. 1964; 3:18-26. DOI: 10.1021/bi00889a005. View

Blair J . A reinterpretation of the kinetics of pyruvate carboxylase. FEBS Lett. 1969; 2(4):245-247. DOI: 10.1016/0014-5793(69)80032-3. View

Dalziel K . The purification of nicotinamide adenine dinucleotide and kinetic effects of nucleotide impurities. J Biol Chem. 1963; 238:1538-43. View

10.

Ning J, Purich D, FROMM H . Studies on the kinetic mechanism and allosteric nature of bovine brain hexokinase. J Biol Chem. 1969; 244(14):3840-6. View

11.

Bachelard H . Allosteric activation of brain hexokinase by magnesium ions and by magnesium ion--adenosine triphosphate complex. Biochem J. 1971; 125(1):249-54. PMC: 1178047. DOI: 10.1042/bj1250249. View

12.

Purich D, FROMM H . Studies on factors influencing enzyme responses to adenylate energy charge. J Biol Chem. 1972; 247(1):249-55. View

13.

Bachelard H, Clark A, Thompson M . Cerebral-cortex hexokinase. Elucidation of reaction mechanisms by substrate and dead-end inhibitor kinetic analysis. Biochem J. 1971; 123(5):707-15. PMC: 1177071. DOI: 10.1042/bj1230707. View

14.

Rose I . The state of magnesium in cells as estimated from the adenylate kinase equilibrium. Proc Natl Acad Sci U S A. 1968; 61(3):1079-86. PMC: 305438. DOI: 10.1073/pnas.61.3.1079. View

15.

FROMM H, Silverstein E, BOYER P . EQUILIBRIUM AND NET REACTION RATES IN RELATION TO THE MECHANISM OF YEAST HEXOKINASE. J Biol Chem. 1964; 239:3645-52. View

16.

FROMM H, Ning J . Kinetic studies of solubilized brain hexokinase with D-fructose as a substrate. Biochem Biophys Res Commun. 1968; 32(4):672-7. DOI: 10.1016/0006-291x(68)90291-x. View

17.

Uyeda K . Studies on the reaction mechanism of skeletal muscle phosphofructokinase. J Biol Chem. 1970; 245(9):2268-75. View

18.

Good N, Winget G, Winter W, Connolly T, Izawa S, Singh R . Hydrogen ion buffers for biological research. Biochemistry. 1966; 5(2):467-77. DOI: 10.1021/bi00866a011. View

19.

Purich D, FROMM H . The kinetics and regulation of rat brain hexokinase. J Biol Chem. 1971; 246(11):3456-63. View

20.

Hill A . The Combinations of Haemoglobin with Oxygen and with Carbon Monoxide. I. Biochem J. 1913; 7(5):471-80. PMC: 1550542. DOI: 10.1042/bj0070471. View