Genetic Analysis of Repair of Ultraviolet Damage by Competent and Noncompetent Cells of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Jan 1

PMID 4630515

Citations 4

Authors

C T Hadden

D BILLEN

Affiliations

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Abstract

The repair of ultraviolet (UV) damage in Bacillus subtilis W23T(-) has been studied by transformation with deoxyribonucleic acid (DNA) extracted from irradiated cells before and after repair. The extent of repair of genetic markers by donor cells after low or moderate doses of UV was found to be related only to the initial degree of inactivation. After a very high dose, further inactivation occurred, also in proportion to initial damage. In addition, the competent recipient cells were shown to repair approximately 75% of the damage in transforming DNA. The sensitivities of markers irradiated either in vivo or in vitro appeared to be related to map position, the more proximal markers showing a greater resistance to UV inactivation.

Citing Articles

Postreplication repair of ultraviolet-irradiated transforming deoxyribonucleic acid in Bacillus subtilis.

Hadden C J Bacteriol. 1981; 145(1):434-41.

PMID: 6780518 PMC: 217291. DOI: 10.1128/jb.145.1.434-441.1981.

The role of the HCR system in the repair of lethal lesions of Bacillus subtilis phages and their transfecting DNA damaged by radiation and alkylating agents.

Vizdalova M, JANOVSKA E, Zhestyanikov V Folia Microbiol (Praha). 1980; 25(5):369-80.

PMID: 6776018 DOI: 10.1007/BF02876689.

Postirradiation recovery dependent on the uvr-1 locus in Bacillus subtilis.

Hadden C J Bacteriol. 1976; 128(1):317-24.

PMID: 824273 PMC: 232858. DOI: 10.1128/jb.128.1.317-324.1976.

Repair and subsequent fragmentation of deoxyribonucleic acid in ultraviolet-irradiated Bacillus subtilis recA.

Hadden C J Bacteriol. 1977; 132(3):856-61.

PMID: 411783 PMC: 235588. DOI: 10.1128/jb.132.3.856-861.1977.

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