Modulation of Cell Behavior in Vitro by the Substratum in Fibroblastic and Leukemic Mouse Cell Lines

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Sep 1

PMID 4627029

Citations 9

Authors

A Macieira-Coelho

S Avrameas

Affiliations

Soon will be listed here.

Abstract

Fibroblastic and leukemic mouse cell lines were cultivated on a bovine serum albumin polymer covered with basic or acidic substances. Contact inhibition of fibroblast movement (cell overlapping) and division varied depending on prior treatment of the albumin polymer. The leukemic cells, which normally grow in suspension, attached and spread out on the polymers and could be carried as a monolayer. Preparation of surfaces capable of modulating different parameters of cell behavior in vitro is thus possible.

Citing Articles

Isolation and Culture of Primary Fibroblasts from Neonatal Murine Hearts to Study Cardiac Fibrosis.

Kumar S, Nagesh D, Ramasubbu V, Bangalore Prabhashankar A, Sundaresan N Bio Protoc. 2023; 13(4):e4616.

PMID: 36845532 PMC: 9947550. DOI: 10.21769/BioProtoc.4616.

Inability of mesoderm cells to locomote on the modified free surface of epithelial cell sheets in vitro.

Sanders E In Vitro. 1982; 18(1):71-8.

PMID: 7182349 DOI: 10.1007/BF02796387.

Modulation of differentiation in vitro. II. Influence of cell spreading and surface events on myogenesis.

Senechal H, Wahrmann J, Delain D, Macieira-Coelho A In Vitro. 1984; 20(9):692-8.

PMID: 6500608 DOI: 10.1007/BF02618874.

Optimal use of the cationic polyelectrolyte poly-L-lysine in the preparation of cell monolayers for diagnostic cytopathology.

Watts K, Husain O J Clin Pathol. 1984; 37(7):829-31.

PMID: 6378981 PMC: 498820. DOI: 10.1136/jcp.37.7.829.

Inhibition of fibronectin receptor function by antibodies against baby hamster kidney cell wheat germ agglutinin receptors.

Grinnell F J Cell Biol. 1982; 95(3):876-84.

PMID: 6296159 PMC: 2112912. DOI: 10.1083/jcb.95.3.876.

References

RYSER H, Hancock R . Histones and basic polyamino acids stimulate the uptake of albumin by tumor cells in culture. Science. 1965; 150(3695):501-3. DOI: 10.1126/science.150.3695.501. View

Todaro G, Lazar G, Green H . The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965; 66(3):325-33. DOI: 10.1002/jcp.1030660310. View

KRUSE Jr P, Miedema E . Production and characterization of multiple-layered populations of animal cells. J Cell Biol. 1965; 27(2):273-9. PMC: 2106720. DOI: 10.1083/jcb.27.2.273. View

Carter S . Principles of cell motility: the direction of cell movement and cancer invasion. Nature. 1965; 208(5016):1183-7. DOI: 10.1038/2081183a0. View

Macieira-Coelho A . Dissociation between inhibition of movement and inhibition of division in RSV transformed human fibroblasts. Exp Cell Res. 1967; 47(1):193-200. DOI: 10.1016/0014-4827(67)90222-4. View

Macieira-Coelho A . Relationship between DNA synthesis and cell density in normal and virus-transformed cells. Int J Cancer. 1967; 2(4):297-303. DOI: 10.1002/ijc.2910020404. View

Baugh C, Lecher R, TYTELL A . The effect of pH on the propagation of the diploid cell WI-38 in galactose medium. J Cell Physiol. 1967; 70(2):225-8. DOI: 10.1002/jcp.1040700213. View

Castor L . Flattening, movement and control of division of epithelial-like cells. J Cell Physiol. 1970; 75(1):57-64. DOI: 10.1002/jcp.1040750107. View

DULBECCO R . Topoinhibition and serum requirement of transformed and untransformed cells. Nature. 1970; 227(5260):802-6. DOI: 10.1038/227802a0. View

10.

Eagle H, FOLEY G, KOPROWSKI H, Lazarus H, Levine E, Adams R . Growth characteristics of virus-transformed cells. Maximum population density, inhibition by normal cells, serum requirement, growth in soft agar, and xenogeneic transplantability. J Exp Med. 1970; 131(4):863-79. PMC: 2138771. DOI: 10.1084/jem.131.4.863. View

11.

Ceccarini C, Eagle H . pH as a determinant of cellular growth and contact inhibition. Proc Natl Acad Sci U S A. 1971; 68(1):229-33. PMC: 391201. DOI: 10.1073/pnas.68.1.229. View

12.

Rubin H . pH and population density in the regulation of animal cell multiplication. J Cell Biol. 1971; 51(3):686-702. PMC: 2108048. DOI: 10.1083/jcb.51.3.686. View

13.

Macieira-Coelho A, Avrameas S . Variations in cell overlapping and growth depending on the substratum. Proc Soc Exp Biol Med. 1972; 139(4):1374-8. DOI: 10.3181/00379727-139-36366. View

14.

ABERCROMBIE M, HEAYSMAN J . Observations on the social behaviour of cells in tissue culture. II. Monolayering of fibroblasts. Exp Cell Res. 1954; 6(2):293-306. DOI: 10.1016/0014-4827(54)90176-7. View