Unrelatedness of Temperate Bacillus Subtilis Bacteriophages SP02 and Phi105

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1972 May 1

PMID 4623614

Citations 25

Authors

L Rutberg

R W Armentrout

J Jonasson

Affiliations

Soon will be listed here.

Abstract

SPO2 and phi105 are temperate Bacillus subtilis bacteriophages which have been suggested to belong to a cluster of related bacteriophages. In the present work, we show that SPO2 does not complement any of the 11 essential genes known in phi105 and that the phages do not recombine. Deoxyribonucleic acid (DNA)-DNA hybridization shows less than 10% homology between SPO2 and phi105 DNA. DNA synthesis in phi105 shows a greater dependence on host functions than does SPO2 DNA synthesis. Growth of phi105 but not of SPO2 is inhibited by the uracil analogue 6-(p-hydroxyphenylazo)-uracil. Infection of a DNA polymerase-deficient strain of B. subtilis with SPO2 leads to an increase in DNA polymerase activity in crude extracts, whereas no such increase is found after infection of this strain with phi105. It is concluded that SPO2 and phi105 are unrelated bacteriophages.

Citing Articles

Transductional selection of cloned bacteriophage phi 105 and SP02 deoxyribonucleic acids in Bacillus subtilis.

Marrero R, Lovett P J Bacteriol. 1980; 143(2):879-86.

PMID: 6782082 PMC: 294385. DOI: 10.1128/jb.143.2.879-886.1980.

Cloning and expression of bacteriophage SP02 DNAZ polymerase gene L in Bacillus subtilis, using the Staphylococcus aureus plasmid pC194.

Rutberg L, Raden B, Flock J J Virol. 1981; 39(2):407-12.

PMID: 6268832 PMC: 171349. DOI: 10.1128/JVI.39.2.407-412.1981.

Nucleotide sequence of the temperate Bacillus subtilis bacteriophage SPO2 DNA polymerase gene L.

Raden B, Rutberg L J Virol. 1984; 52(1):9-15.

PMID: 6090713 PMC: 254482. DOI: 10.1128/JVI.52.1.9-15.1984.

Characterization of interspecific plasmid transfer mediated by Bacillus subtilis temperate bacteriophage SP02.

Marrero R, Young F, Yasbin R J Bacteriol. 1984; 160(1):458-61.

PMID: 6090434 PMC: 214745. DOI: 10.1128/jb.160.1.458-461.1984.

Transformation and transfection in lysogenic strains of Bacillus subtilis 168.

Yasbin R, Wilson G, Young F J Bacteriol. 1973; 113(2):540-8.

PMID: 4632315 PMC: 285263. DOI: 10.1128/jb.113.2.540-548.1973.

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