Bacillus Subtilis DNA Polymerase III is Required for the Replication of the Virulent Bacteriophage Phi E

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1974 Dec 1

PMID 4214945

Citations 5

Authors

U Lavi

A Nattenberg

A Ronen

M Marcus

Affiliations

Soon will be listed here.

Abstract

The virulent phage phie of Bacillus subtilis which contains hydroxymethyluracil in its DNA requires host DNA polymerase III for its DNA replication. DNA polymerase III(ts) mutant cells infected with phie at restrictive temperatures do not support phage DNA synthesis. However, phie grows normally both at low and high temperatures in the mutant's parent strain and in spontaneous DNA polymerase III(+) revertants isolated from the mutant strain. Temperature-shift-down experiments with phie-infected cells having thermosensitive DNA polymerase III (pol III(ts)) indicate that at 48 C the thermolabile DNA polymerase III is irreversibly inactivated and has to be synthesized de novo after the shift to 37 C, before phage DNA synthesis can begin. Temperature-shift-up experiments with phie-infected mutant cells show that phage replication is arrested immediately after the temperature shift and indicate that phie requires DNA polymerase III throughout its replication stage.

Citing Articles

Action of 6-(p-hydroxyphenylazo)-uracil on bacteriophage IG 1.

Fernandes R, de Lencastre H, Archer L Arch Virol. 1990; 113(3-4):177-81.

PMID: 2121120 DOI: 10.1007/BF01316671.

Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III.

Montenegro M, Esche H, Trautner T Mol Gen Genet. 1976; 149(2):131-4.

PMID: 827685 DOI: 10.1007/BF00332880.

Bacteriophages of Bacillus subtilis.

Hemphill H, WHITELEY H Bacteriol Rev. 1975; 39(3):257-315.

PMID: 809034 PMC: 413919. DOI: 10.1128/br.39.3.257-315.1975.

Bacillus subtilis DNA polymerase III is required for the replication of DNA of bacteriophages SPP-1 and phi 105.

Rowley S, Brown N J Virol. 1977; 21(2):493-6.

PMID: 401898 PMC: 353849. DOI: 10.1128/JVI.21.2.493-496.1977.

Relationship of Bacillus subtilis DNA polymerase III to bacteriophage PBS2-induced DNA polymerase and to the replication of uracil-containing DNA.

Hitzeman R, Price A J Virol. 1978; 28(3):697-709.

PMID: 104052 PMC: 525793. DOI: 10.1128/JVI.28.3.697-709.1978.

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