Topography of Escherichia Coli Ribosomal Proteins. The Order of Reactivity of Thiol Groups

Overview

Journal Biochem J

Specialty Biochemistry

Date 1974 Dec 1

PMID 4618476

Citations 1

Authors

A Bakardjieva

R R Crichton

Affiliations

Soon will be listed here.

Abstract

1. 30S and 50S ribosomal subunits of Escherichia coli were treated with N-[2,3-(14)C]-ethylmaleimide and iodo[(14)C]acetamide. 2. The proteins in the native subunits which reacted with the reagents were S1,double dagger S2, S12, S13, S18, S21, L2, L5, L6, L10, L11, L15, L17, L20, L26+28 and L27. 3. Several proteins, such as S1, S12, S14, S18, L2, L6, L10, L11 and either L26 or 28, had thiol groups in an oxidized form and reacted to a greater extent after reduction with beta-mercaptoethanol or dithiothreitol. 4. The total number of thiol groups in 30S and 50S subunits was determined as 16-17 and 26-27 respectively. The total number of thiol groups in each ribosomal protein was also determined. 5. The reaction of 30S and 50S subunits with iodoacetamide under several different conditions established the order of reactivity of thiol groups.

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References

GESTELAND R . Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966; 16(1):67-84. DOI: 10.1016/s0022-2836(66)80263-2. View

SPAHR P . Amino acid composition of ribosomes from Escherichia coli. J Mol Biol. 1962; 4:395-406. DOI: 10.1016/s0022-2836(62)80020-5. View

Traut R, Haenni A . The effect of sulfhydryl reagents on ribosome activity. Eur J Biochem. 1967; 2(1):4-73. DOI: 10.1111/j.1432-1033.1967.tb00107.x. View

HEINTZ R, McAllister H, Arlinghaus R, Schweet R . Formation and function of the active ribosome complex. Cold Spring Harb Symp Quant Biol. 1966; 31:633-9. DOI: 10.1101/sqb.1966.031.01.082. View

Retsema J, CONWAY T . Reversible dissociation of Escherichia coli ribosomes by N-ethylmaleimide. Biochim Biophys Acta. 1969; 179(2):369-80. DOI: 10.1016/0005-2787(69)90045-8. View

Hardy S, Kurland C, Voynow P, Mora G . The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969; 8(7):2897-905. DOI: 10.1021/bi00835a031. View

Staehelin T, Maglott D, MONRO R . On the catalytic center of peptidyl transfer: a part of the 50 S ribosome structure. Cold Spring Harb Symp Quant Biol. 1969; 34:39-48. DOI: 10.1101/sqb.1969.034.01.008. View

Kaltschmidt E, WITTMANN H . Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970; 36(2):401-12. DOI: 10.1016/0003-2697(70)90376-3. View

Kaltschmidt E, WITTMANN H . Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis. Proc Natl Acad Sci U S A. 1970; 67(3):1276-82. PMC: 283348. DOI: 10.1073/pnas.67.3.1276. View

10.

Voynow P, Kurland C . Stoichiometry of the 30S ribosomal proteins of Escherichia coli. Biochemistry. 1971; 10(3):517-24. DOI: 10.1021/bi00779a026. View

11.

WITTMANN H, Stofflet G, Hindennach I, Kurland C, Birge E, Nomura M . Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories. Mol Gen Genet. 1971; 111(4):327-33. DOI: 10.1007/BF00569784. View

12.

Moore P . Reaction of N-ethyl maleimide with the ribosomes of Escherichia coli. J Mol Biol. 1971; 60(1):169-84. DOI: 10.1016/0022-2836(71)90456-6. View

13.

McAllister H, Schweet R . Involvement of sulfhydryl groups in the binding of tRNA to reticulocyte ribosomes. J Mol Biol. 1968; 34(3):519-25. DOI: 10.1016/0022-2836(68)90177-0. View

14.

SLOBIN L . Sulfhydryl groups of Escherichia coli Ribosomes and ribosomal proteins. J Mol Biol. 1971; 61(1):281-5. DOI: 10.1016/0022-2836(71)90227-0. View

15.

Weber H . Stoichiometric measurements of 30S and 50S ribosomal proteins from Escherichia coli. Mol Gen Genet. 1972; 119(3):233-48. DOI: 10.1007/BF00333861. View

16.

Acharya A, Moore P . Reaction of ribosomal sulfhydryl groups with 5,5'-dithiobis(2-nitrobenzoic acid). J Mol Biol. 1973; 76(2):207-21. DOI: 10.1016/0022-2836(73)90385-9. View

17.

Moore G, Crichton R . Reductive methylation: a method for preparing functionally active radioactive ribosomes. FEBS Lett. 1973; 37(1):74-8. DOI: 10.1016/0014-5793(73)80429-6. View

18.

Reinbolt J, Schiltz E . The primary structure of ribosomal protein S4 from Escherichia coli. FEBS Lett. 1973; 36(3):250-2. DOI: 10.1016/0014-5793(73)80383-7. View

19.

Pongs O, Bald R, Erdmann V . Identification of chloramphenicol-binding protein in Escherichia coli ribosomes by affinity labeling. Proc Natl Acad Sci U S A. 1973; 70(8):2229-33. PMC: 433707. DOI: 10.1073/pnas.70.8.2229. View

20.

Wang J, Matheson A . The possible role of sulfhydryl groups in the dimerization of 70S ribosomes from Escherichia coli. Biochem Biophys Res Commun. 1966; 23(5):740-4. DOI: 10.1016/0006-291x(66)90463-3. View