Studies on in Vitro DNA Synthesis: Purification of Dna C Gene Product Containing Dna D Activity from Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 Aug 1

PMID 4599623

Citations 16

Authors

S Wickner

I Berkower

M Wright

J Hurwitz

Affiliations

Soon will be listed here.

Abstract

The conversion of varphiX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The dna C and dna D gene products copurify, have similar characteristics, are both temperature sensitive in preparations from dna C temperature-sensitive cells, and are both undetectable in preparations from dna D temperature-sensitive cells.

Citing Articles

The dnaC protein of Escherichia coli. Purification, physical properties and interaction with dnaB protein.

LANKA E, Schuster H Nucleic Acids Res. 1983; 11(4):987-97.

PMID: 6298736 PMC: 325772. DOI: 10.1093/nar/11.4.987.

dnaC-dependent reconstitution of replication forks in Escherichia coli lysates.

Niedenhof I, Rein R J Bacteriol. 1982; 150(1):286-92.

PMID: 6277866 PMC: 220111. DOI: 10.1128/jb.150.1.286-292.1982.

Replication of the bacteriocinogenic plasmid Clo DF13 in thermosensitive Escherichia coli mutants defective in initiation or elongation of deoxyribonucleic acid replication.

Veltkamp E, Nijkamp H J Bacteriol. 1974; 120(3):1227-37.

PMID: 4612010 PMC: 245905. DOI: 10.1128/jb.120.3.1227-1237.1974.

Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.

Wickner S, Hurwitz J Proc Natl Acad Sci U S A. 1974; 71(10):4120-4.

PMID: 4610569 PMC: 434340. DOI: 10.1073/pnas.71.10.4120.

Temperature-sensitive modification and restriction phenotypes of an Escherichia coli dnaD mutant.

Paul A, Inouye M J Bacteriol. 1974; 119(3):907-12.

PMID: 4605206 PMC: 245698. DOI: 10.1128/jb.119.3.907-912.1974.

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