Studies on Acetyl-coenzyme A Synthetase of Yeast: Inhibition by Long-chain Acyl-coenzyme A Esters

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Aug 1

PMID 4579873

Citations 5

Authors

T Satyanarayana

H P Klein

Affiliations

Soon will be listed here.

Abstract

Long-chain acyl-coenzyme A (CoA) compounds (palmityl, stearyl, and oleyl) were found to be potent inhibitors of acetyl-CoA synthetase (ACS) of Saccharomyces cerevisiae strain LK2G12 from aerobic, but not from nonaerobic, cells. The effectiveness of the inhibitors of the aerobic enzyme was in the following order: palmityl-CoA < stearyl-CoA < oleyl-CoA. Short-chain acyl-CoA compounds (propionyl, butyryl, and valeryl) and long-chain fatty acids had no effect on ACS from either source. The inhibition by oleyl-CoA was found to be dependent on enzyme concentration, whereas the inhibition by palmityl- and stearyl-CoA was independent of ACS concentration. Inhibition by palmityl-CoA was noncompetitive with respect to both acetate and CoA, and with increasing concentration of inhibitor the pattern was sigmoidal, with a Hill value of 3.24. At maximally inhibitory concentrations of palmityl-CoA, a small amount of enzyme activity remained. This noninhibitable enzyme in aerobic cells was shown not to be of nonaerobic origin.

Citing Articles

Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance.

Ding J, Holzwarth G, Penner M, Patton-Vogt J, Bakalinsky A FEMS Microbiol Lett. 2015; 362(3):1-7.

PMID: 25673654 PMC: 4809976. DOI: 10.1093/femsle/fnu042.

Coordination of the dynamics of yeast sphingolipid metabolism during the diauxic shift.

Alvarez-Vasquez F, Sims K, Voit E, Hannun Y Theor Biol Med Model. 2007; 4:42.

PMID: 17974024 PMC: 2203994. DOI: 10.1186/1742-4682-4-42.

Lipids of yeasts.

Rattray J, Schibeci A, Kidby D Bacteriol Rev. 1975; 39(3):197-231.

PMID: 240350 PMC: 413914. DOI: 10.1128/br.39.3.197-231.1975.

Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes.

Jauniaux J, Urrestarazu L, Wiame J J Bacteriol. 1978; 133(3):1096-1107.

PMID: 205532 PMC: 222139. DOI: 10.1128/jb.133.3.1096-1107.1978.

Effects of aeration on formation and localization of the acetyl coenzyme A synthetases of Saccharomyces cerevisiae.

Klein H, JAHNKE L J Bacteriol. 1979; 137(1):179-84.

PMID: 33146 PMC: 218433. DOI: 10.1128/jb.137.1.179-184.1979.

References

Kornberg A, PRICER Jr W . Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids. J Biol Chem. 1953; 204(1):329-43. View

PANDE S, Mead J . Inhibition of enzyme activities by free fatty acids. J Biol Chem. 1968; 243(23):6180-5. View

Grunert R, Phillips P . A modification of the nitroprusside method of analysis for glutathione. Arch Biochem. 1951; 30(2):217-25. View

White D, Klein H . FACTORS AFFECTING FATTY ACID SYNTHESIS IN CELL-FREE PREPARATIONS FROM SACCHAROMYCES CEREVISIAE. Biochem Biophys Res Commun. 1965; 20:78-84. DOI: 10.1016/0006-291x(65)90953-8. View

Eaton N, Klein H . Studies on the aerobic degradation of glucose by Saccharomyces cerevisiae. Biochem J. 1957; 67(3):373-81. PMC: 1200167. DOI: 10.1042/bj0670373. View

Wojtczak L, ZALUSKA H . The inhibition of translocation of adenine nucleotides through mitochondrial membranes by oleate. Biochem Biophys Res Commun. 1967; 28(1):76-81. DOI: 10.1016/0006-291x(67)90409-3. View

Lynen F . The role of biotin-dependent carboxylations in biosynthetic reactions. Biochem J. 1967; 102(2):381-400. PMC: 1270259. DOI: 10.1042/bj1020381. View

Klein H, JAHNKE L . Cellular localization of acetyl-coenzyme A synthetase in yeast. J Bacteriol. 1968; 96(5):1632-9. PMC: 315221. DOI: 10.1128/jb.96.5.1632-1639.1968. View

Taketa K, POGELL B . The effect of palmityl coenzyme A on glucose 6-phosphate dehydrogenase and other enzymes. J Biol Chem. 1966; 241(3):720-6. View

10.

Tubbs P, Garland P . Variations in tissue contents of coenzyme A thio esters and possible metabolic implications. Biochem J. 1964; 93(3):550-7. PMC: 1214008. DOI: 10.1042/bj0930550. View

11.

SRERE P . Palmityl-coenzyme A inhibition of the citrate-condensing enzyme. Biochim Biophys Acta. 1965; 106(3):445-55. DOI: 10.1016/0005-2760(65)90061-5. View

12.

Rydstrom J, Panov A, Paradies G, Ernster L . Inhibition of mitochondrial nicotinamide nucleotide transhydrogenase by CoA-thioesters of long-chain fatty acids. Biochem Biophys Res Commun. 1971; 45(6):1389-97. DOI: 10.1016/0006-291x(71)90175-6. View

13.

Klein H . Some observations on a cell free lipid synthesizing system from Saccharomyces cerevisiae. J Bacteriol. 1957; 73(4):530-43. PMC: 314614. DOI: 10.1128/jb.73.4.530-537.1957. View

14.

Berg P . Acyl adenylates; an enzymatic mechanism of acetate activation. J Biol Chem. 1956; 222(2):991-1013. View

15.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

16.

Klein H, JAHNKE L . Variations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells. J Bacteriol. 1971; 106(2):596-602. PMC: 285135. DOI: 10.1128/jb.106.2.596-602.1971. View