Arginine Metabolism in Saccharomyces Cerevisiae: Subcellular Localization of the Enzymes

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1978 Mar 1

PMID 205532

Citations 57

Authors

J C Jauniaux

L A Urrestarazu

J M Wiame

Affiliations

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Abstract

Subcellular localization of enzymes of arginine metabolism in Saccharomyces cerevisiae was studied by partial fractionation and stepwise homogenization of spheroplast lysates. These enzymes could clearly be divided into two groups. The first group comprised the five enzymes of the acetylated compound cycle, i.e., acetylglutamate synthase, acetylglutamate kinase, acetylglutamyl-phosphate reductase, acetylornithine aminotransferase, and acetylornithine-glutamate acetyltransferase. These enzymes were exclusively particulate. Comparison with citrate synthase and cytochrome oxidase, and results from isopycnic gradient analysis, suggested that these enzymes were associated with the mitochondria. By contrast, enzymatic activities going from ornithine to arginine, i.e., arginine pathway-specific carbamoylphosphate synthetase, ornithine carbamoyltransferase, argininosuccinate synthetase, and argininosuccinate lyase, and the two first catabolic enzymes, arginase and ornithine aminotransferase, were in the "soluble" fraction of the cell.

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References

Satyanarayana T, Klein H . Studies on acetyl-coenzyme A synthetase of yeast: inhibition by long-chain acyl-coenzyme A esters. J Bacteriol. 1973; 115(2):600-6. PMC: 246288. DOI: 10.1128/jb.115.2.600-606.1973. View

Hollenberg C, Riks W, Borst P . The glutamate dehydrogenases of yeast: extra-mitochondrial enzymes. Biochim Biophys Acta. 1970; 201(1):13-9. DOI: 10.1016/0304-4165(70)90004-8. View

Boller T, Durr M, Wiemken A . Characterization of a specific transport system for arginine in isolated yeast vacuoles. Eur J Biochem. 1975; 54(1):81-91. DOI: 10.1111/j.1432-1033.1975.tb04116.x. View

Shigesada K, TATIBANA M . Enzymatic synthesis of acetylglutamate by mammalian liver preparations and its stimulation by arginine. Biochem Biophys Res Commun. 1971; 44(5):1117-24. DOI: 10.1016/s0006-291x(71)80201-2. View

Smith L . A study of some oxidative enzymes of baker's yeast. Arch Biochem Biophys. 1954; 50(2):285-98. DOI: 10.1016/0003-9861(54)90044-2. View

Wiemken A, Durr M . Characterization of amino acid pools in the vacuolar compartment of Saccharomyces cerevisiae. Arch Microbiol. 1974; 101(1):45-57. DOI: 10.1007/BF00455924. View

Urrestarazu L, Vissers S, Wiame J . Change in location of ornithine carbamoyltransferase and carbamoylphosphate synthetase among yeasts in relation to the arginase/ornithine carbamoyltransferase regulatory complex and the energy status of the cells. Eur J Biochem. 1977; 79(2):473-81. DOI: 10.1111/j.1432-1033.1977.tb11830.x. View

DE DEKEN R . Pathway of arginine biosynthesis in yeast. Biochem Biophys Res Commun. 1962; 8:462-6. DOI: 10.1016/0006-291x(62)90297-8. View

Glansdorff N . TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12. Genetics. 1965; 51:167-79. PMC: 1210772. DOI: 10.1093/genetics/51.2.167. View

10.

Bowman B, Davis R . Arginine catabolism in Neurospora: cycling of ornithine. J Bacteriol. 1977; 130(1):285-91. PMC: 235204. DOI: 10.1128/jb.130.1.285-291.1977. View

11.

Satyanarayana T, Klein H . Studies on the "aerobic" acetyl-coenzyme A synthetase of Saccharomyces cerevisiae: purification, crystallization, and physical properties of the enzyme. Arch Biochem Biophys. 1975; 174(2):480-90. DOI: 10.1016/0003-9861(76)90376-3. View

12.

Lacroute F, PIERARD A, GRENSON M, Wiame J . The biosynthesis of carbamoyl phosphate in Saccharomyces cerevisiae. J Gen Microbiol. 1965; 40(1):127-42. DOI: 10.1099/00221287-40-1-127. View

13.

Bowman B, Davis R . Cellular distribution of ornithine in Neurospora: anabolic and catabolic steady states. J Bacteriol. 1977; 130(1):274-84. PMC: 235203. DOI: 10.1128/jb.130.1.274-284.1977. View

14.

Penninckx M, Wiame J . Affinity of arginase for ornithine carbamoyltransferase in Saccharomyces cerevisiae. J Mol Biol. 1976; 104(4):819-31. DOI: 10.1016/0022-2836(76)90184-4. View

15.

Davis R . Metabolite distribution in cells. Science. 1972; 178(4063):835-40. DOI: 10.1126/science.178.4063.835. View

16.

Gamble J, Lehninger A . Transport of ornithine and citrulline across the mitochondrial membrane. J Biol Chem. 1973; 248(2):610-8. View

17.

Delforge J, Messenguy F, Wiame J . The regulation of arginine biosynthesis in Saccharomyces cerevisiae. The specificity of argR- mutations and the general control of amino-acid biosynthesis. Eur J Biochem. 1975; 57(1):231-9. DOI: 10.1111/j.1432-1033.1975.tb02295.x. View

18.

Bechet J, Greenson M, Wiame J . Mutations affecting the repressibility of arginine biosynthetic enzymes in Saccharomyces cerevisiae. Eur J Biochem. 1970; 12(1):31-9. DOI: 10.1111/j.1432-1033.1970.tb00817.x. View

19.

DUELL E, Inoue S, UTTER M . ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST. J Bacteriol. 1964; 88:1762-73. PMC: 277483. DOI: 10.1128/jb.88.6.1762-1773.1964. View

20.

Perlman P, Mahler H . Intracellular localization of enzymes in yeast. Arch Biochem Biophys. 1970; 136(1):245-59. DOI: 10.1016/0003-9861(70)90348-6. View