Cell Wall Peptidoglycan Mutants of Escherichia Coli K-12: Existence of Two Clusters of Genes, Mra and Mrb, for Cell Wall Peptidoglycan Biosynthesis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Nov 1

PMID 4563986

Citations 50

Authors

T Miyakawa

H Matsuzawa

M Matsuhashi

Y Sugino

Affiliations

Soon will be listed here.

Abstract

Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis.

Citing Articles

Taxonomic identification and temperature stress tolerance mechanisms of Aequorivita marisscotiae sp. nov.

Liu W, Cong B, Lin J, Liu S, Deng A, Zhao L Commun Biol. 2023; 6(1):1186.

PMID: 37990058 PMC: 10663628. DOI: 10.1038/s42003-023-05559-7.

Unipolar Peptidoglycan Synthesis in the Requires an Essential Class A Penicillin-Binding Protein.

Williams M, Aliashkevich A, Krol E, Kuru E, Bouchier J, Rittichier J mBio. 2021; 12(5):e0234621.

PMID: 34544272 PMC: 8546619. DOI: 10.1128/mBio.02346-21.

Identification and Partial Characterization of a Novel UDP-N-Acetylenolpyruvoylglucosamine Reductase/UDP-N-Acetylmuramate:l-Alanine Ligase Fusion Enzyme from Verrucomicrobium spinosum DSM 4136(T).

Naqvi K, Patin D, Wheatley M, Savka M, Dobson R, Gan H Front Microbiol. 2016; 7:362.

PMID: 27047475 PMC: 4803751. DOI: 10.3389/fmicb.2016.00362.

Mutation of the murC and murB Genes Impairs Heterocyst Differentiation in Anabaena sp. Strain PCC 7120.

Videau P, Rivers O, Ushijima B, Oshiro R, Kim M, Philmus B J Bacteriol. 2016; 198(8):1196-206.

PMID: 26811320 PMC: 4859589. DOI: 10.1128/JB.01027-15.

Lipid Flippases for Bacterial Peptidoglycan Biosynthesis.

Ruiz N Lipid Insights. 2016; 8(Suppl 1):21-31.

PMID: 26792999 PMC: 4714577. DOI: 10.4137/LPI.S31783.

References

Curtiss 3rd R, Charamella L, Berg C, Harris P . Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli. J Bacteriol. 1965; 90(5):1238-50. PMC: 315808. DOI: 10.1128/jb.90.5.1238-1250.1965. View

Strominger J, Izaki K, Matsuhashi M, Tipper D . Peptidoglycan transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions. Fed Proc. 1967; 26(1):9-22. View

Anderson J, Matsuhashi M, HASKIN M, Strominger J . Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence. J Biol Chem. 1967; 242(13):3180-90. View

Izaki K, Matsuhashi M, Strominger J . Biosynthesis of the peptidoglycan of bacterial cell walls. 8. Peptidoglycan transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reaction in strains of Escherichia coli. J Biol Chem. 1968; 243(11):3180-92. View

Yura T, Wada C . Phenethyl alcohol resistance in Escherichia coli. I. Resistance of strain C600 and its relation to azide resistance. Genetics. 1968; 59(2):177-90. PMC: 1211940. DOI: 10.1093/genetics/59.2.177. View

Matsuzawa H, Matsuhashi M, Oka A, Sugino Y . Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12. Biochem Biophys Res Commun. 1969; 36(4):682-9. DOI: 10.1016/0006-291x(69)90360-x. View

Taylor A . Current linkage map of Escherichia coli. Bacteriol Rev. 1970; 34(2):155-75. PMC: 408314. DOI: 10.1128/br.34.2.155-175.1970. View

Endo A, Kakiki K, Hori M, Abe H, MISATO T . A simple procedure for the preparation of UDP-N-acetylglucosamine-14C. Biochem Biophys Res Commun. 1970; 39(4):718-22. DOI: 10.1016/0006-291x(70)90264-0. View

Bukhari A, Taylor A . Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli. J Bacteriol. 1971; 105(3):844-54. PMC: 248509. DOI: 10.1128/jb.105.3.844-854.1971. View

10.

Tamaki S, Sato T, Matsuhashi M . Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12. J Bacteriol. 1971; 105(3):968-75. PMC: 248525. DOI: 10.1128/jb.105.3.968-975.1971. View

11.

Blumberg P, Strominger J . Inactivation of D-alanine carboxypeptidase by penicillins and cephalosporins is not lethal in Bacillus subtilis. Proc Natl Acad Sci U S A. 1971; 68(11):2814-7. PMC: 389532. DOI: 10.1073/pnas.68.11.2814. View

12.

Lugtenberg E, Ruyters W . Murein synthesis and identification of cell wall precursors of temperature-sensitive lysis mutants of Escherichia coli. J Bacteriol. 1972; 109(1):326-35. PMC: 247283. DOI: 10.1128/jb.109.1.326-335.1972. View

13.

Campbell A, DEL CAMPILLO-CAMPBELL A, Chang R . A mutant of Escherichia coli that requires high concentrations of biotin. Proc Natl Acad Sci U S A. 1972; 69(3):676-80. PMC: 426533. DOI: 10.1073/pnas.69.3.676. View

14.

Lugtenberg E . Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme. J Bacteriol. 1972; 110(1):35-40. PMC: 247375. DOI: 10.1128/jb.110.1.35-40.1972. View

15.

Lugtenberg E . Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme. J Bacteriol. 1972; 110(1):41-6. PMC: 247376. DOI: 10.1128/jb.110.1.41-46.1972. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

LEDERBERG J, LEDERBERG E . Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952; 63(3):399-406. PMC: 169282. DOI: 10.1128/jb.63.3.399-406.1952. View

18.

WEIDEL W, Pelzer H . BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS. Adv Enzymol Relat Subj Biochem. 1964; 26:193-232. DOI: 10.1002/9780470122716.ch5. View

19.

Taylor A, THOMAN M . THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964; 50:659-77. PMC: 1210685. DOI: 10.1093/genetics/50.4.659. View

20.

Lennox E . Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955; 1(2):190-206. DOI: 10.1016/0042-6822(55)90016-7. View