Genetic Analysis of Diaminopimelic Acid- and Lysine-requiring Mutants of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Mar 1

PMID 4926684

Citations 75

Authors

A I Bukhari

A L Taylor

Affiliations

Soon will be listed here.

Abstract

Several diaminopimelic acid (DAP)- and lysine-requiring mutants of Escherichia coli were isolated and studied by genetic, physiological, and biochemical means. The genes concerned with DAP-lysine synthesis map at several different sites on the E. coli chromosome and, therefore, do not constitute a single operon. Three separate loci affecting DAP synthesis are located in the 0 to 2.5 min region of the genetic map. The order of the loci in this region is thr-dapB-pyrA-ara-leu-pan-dapC-tonA-dapD. Two additional DAP genes map in the region between min 47 and 48, with the gene order being gua-dapA-dapE-ctr. The lys locus at min 55 determines the synthesis of the enzyme DAP decarboxylase, which catalyzes the conversion of DAP into lysine. The order of the genes in this region is serA-lysA-thyA.

Citing Articles

Replacing rice straw with peanut vine and silage in beef cattle feed reduced the use of soybean meal.

Yi X, Li Y, Liu Y, Zhang M, Zhou Z, Meng Q Anim Nutr. 2025; 20:42-53.

PMID: 39949735 PMC: 11821392. DOI: 10.1016/j.aninu.2024.11.013.

Conjugal DNA Transfer in Sodalis glossinidius, a Maternally Inherited Symbiont of Tsetse Flies.

Kendra C, Keller C, Bruna R, Pontes M mSphere. 2020; 5(6).

PMID: 33148821 PMC: 7643829. DOI: 10.1128/mSphere.00864-20.

Competing Substrates for the Bifunctional Diaminopimelic Acid Epimerase/Glutamate Racemase Modulate Peptidoglycan Synthesis in Chlamydia trachomatis.

Singh R, Slade J, Brockett M, Mendez D, Liechti G, Maurelli A Infect Immun. 2020; 89(1).

PMID: 33106295 PMC: 7927921. DOI: 10.1128/IAI.00401-20.

A Physiological Basis for Nonheritable Antibiotic Resistance.

Pontes M, Groisman E mBio. 2020; 11(3).

PMID: 32546621 PMC: 7298711. DOI: 10.1128/mBio.00817-20.

Slow growth determines nonheritable antibiotic resistance in .

Pontes M, Groisman E Sci Signal. 2019; 12(592).

PMID: 31363068 PMC: 7206539. DOI: 10.1126/scisignal.aax3938.

References

White P, Kelly B, Suffling A, WORK E . Variation of activity of bacterial diaminopimelate decarboxylase under different conditions of growth. Biochem J. 1964; 91(3):600-10. PMC: 1202998. DOI: 10.1042/bj0910600. View

PATTE J, COHEN G . [Isolation and properties of a lysine-sensitive mutant of Escherichia coli devoid of aspartokinase]. Biochim Biophys Acta. 1965; 99(3):561-3. View

YUGARI Y, Gilvarg C . The condensation step in diaminopimelate synthesis. J Biol Chem. 1965; 240(12):4710-6. View

FARKAS W, Gilvarg C . The reduction step in diaminopimelic acid biosynthesis. J Biol Chem. 1965; 240(12):4717-22. View

MARTIN H . Biochemistry of bacterial cell walls. Annu Rev Biochem. 1966; 35:457-84. DOI: 10.1146/annurev.bi.35.070166.002325. View

Schwartz M . Location of the maltose A and B loci on the genetic map of Escherichia coli. J Bacteriol. 1966; 92(4):1083-9. PMC: 276382. DOI: 10.1128/jb.92.4.1083-1089.1966. View

Stadtman E . Allosteric regulation of enzyme activity. Adv Enzymol Relat Areas Mol Biol. 1966; 28:41-154. DOI: 10.1002/9780470122730.ch2. View

DEMEREC M, Adelberg E, Clark A, Hartman P . A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966; 54(1):61-76. PMC: 1211113. DOI: 10.1093/genetics/54.1.61. View

Taylor A, Trotter C . Revised linkage map of Escherichia coli. Bacteriol Rev. 1967; 31(4):332-53. PMC: 408288. DOI: 10.1128/br.31.4.332-353.1967. View

10.

Fukuda A, Gilvarg C . The relationship of dipicolinate and lysine biosynthesis in Bacillus megaterium. J Biol Chem. 1968; 243(14):3871-6. View

11.

Truffa-Bachi P, COHEN G . Some aspects of amino acid biosynthesis in microorganisms. Annu Rev Biochem. 1968; 37:79-108. DOI: 10.1146/annurev.bi.37.070168.000455. View

12.

Normark S, Boman H, Matsson E . Mutant of Escherichia coli with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics. J Bacteriol. 1969; 97(3):1334-42. PMC: 249852. DOI: 10.1128/jb.97.3.1334-1342.1969. View

13.

Wang R, Morse H, Morse M . Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations. J Bacteriol. 1969; 98(2):605-10. PMC: 284861. DOI: 10.1128/jb.98.2.605-610.1969. View

14.

Barnes I, Bondi A, Moat A . Biochemical characterization of lysine auxotrophs of Staphylococcus aureus. J Bacteriol. 1969; 99(1):169-74. PMC: 249983. DOI: 10.1128/jb.99.1.169-174.1969. View

15.

Matsuzawa H, Matsuhashi M, Oka A, Sugino Y . Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12. Biochem Biophys Res Commun. 1969; 36(4):682-9. DOI: 10.1016/0006-291x(69)90360-x. View

16.

Tritz G, Matney T, Gholson R . Mapping of the nadB locus adjacent to a previously undescribed purine locus in Escherichia coli K-12. J Bacteriol. 1970; 102(2):377-81. PMC: 247561. DOI: 10.1128/jb.102.2.377-381.1970. View

17.

Taylor A . Current linkage map of Escherichia coli. Bacteriol Rev. 1970; 34(2):155-75. PMC: 408314. DOI: 10.1128/br.34.2.155-175.1970. View

18.

Bukhari A, Taylor A . Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine. J Bacteriol. 1971; 105(3):988-98. PMC: 248528. DOI: 10.1128/jb.105.3.988-998.1971. View

19.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

20.

Salton M . Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim Biophys Acta. 1953; 10(4):512-23. DOI: 10.1016/0006-3002(53)90296-0. View