Isolation of Transducing Bacteriophages for the Histidine and Isoleucine-valine Operons in Escherichia Coli K-12

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Oct 1

PMID 4562404

Citations 23

Authors

A Avitabile

R Favvre

F Blasi

Affiliations

Soon will be listed here.

Abstract

In vitro studies have been of great value in elucidating the mechanism of the regulation of several bacterial operons. To obtain a deoxyribonucleic acid preparation enriched for the histidine (his) and for the isoleucine-valine (ilv) operons, we have isolated bacteriophages carrying the his and the ilv regions of the Escherichia coli chromosome. Transposition of the his operon to a site close to the att80 region of the E. coli chromosome has been carried out selecting for integration of a temperature-sensitive F'his(+) in the tonB locus. This transposed strain has been lysogenized with phi80i(lambda). Upon induction of the lysogen, His(+) transductants have been isolated, which, on further induction give rise to HFT (high frequency of transduction) lysates. Preliminary characterization of the transducing phage is reported. The ilv operon, carried on an F' particle, has been fused to an episome carrying the att80 region. The fused episome has been lysogenized with phi80i lambdat68. Upon induction of the lysogen, Ilv(+) transductants have been isolated which on further induction give rise to HFT lysates.

Citing Articles

Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli.

Nargang F, SUBRAHMANYAM C, Umbarger H Proc Natl Acad Sci U S A. 1980; 77(4):1823-7.

PMID: 6990415 PMC: 348600. DOI: 10.1073/pnas.77.4.1823.

Cloning and expression of the distal portion of the histidine operon of Escherichia coli K-12.

Grisolia V, Carlomagno M, Bruni C J Bacteriol. 1982; 151(2):692-700.

PMID: 6284708 PMC: 220310. DOI: 10.1128/jb.151.2.692-700.1982.

Structural and physiological studies of the Escherichia coli histidine operon inserted into plasmid vectors.

Bruni C, Musti A, Frunzio R, Blasi F J Bacteriol. 1980; 142(1):32-42.

PMID: 6246067 PMC: 293896. DOI: 10.1128/jb.142.1.32-42.1980.

Isoleucine and valine metabolism in Escherichia coli K-12: detection and measurement of ilv-specific messenger ribonucleic acid.

Vonder Haar R, Umbarger H J Bacteriol. 1974; 120(2):687-96.

PMID: 4616946 PMC: 245828. DOI: 10.1128/jb.120.2.687-696.1974.

Deoxyribonucleic acid-directed in vitro synthesis of ilv-specific messenger ribonucleic acid by extracts of Escherichia coli K-12.

Smolin D, Umbarger H J Bacteriol. 1974; 120(3):1238-48.

PMID: 4612011 PMC: 245906. DOI: 10.1128/jb.120.3.1238-1248.1974.

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