Cellular Immunity to Nuclear Antigens in Systemic Lupus Erythematosus

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1972 Oct 1

PMID 4560340

Citations 10

Authors

J A Goldman

A Litwin

L E Adams

R C KRUEGER

E V Hess

Affiliations

Soon will be listed here.

Abstract

Cellular immune repsonses were determined by skin testing and mitogen- and antigen-induced blastic transformation of peripheral blood lymphocyte cultures in 24 patients with systemic lupus erythematosus (SLE) and 24 normal subjects. The incidence of positive skin tests with Candida albicans, PPD (tuberculin-purified protein derivative) intermediate strength, Trichophyton and histoplasmin was not significantly different in the two groups nor was lymphocyte stimulation by the mitogen phytohemagglutinin-M (PHA-M), implying that cellular immunity is normal in SLE. However, the SLE patients had a significantly increased incidence of positive skin tests and stimulated lymphocyte cultures to a number of nuclear antigens compared with normal subjects. No correlation could be made between the test results and the activity of the SLE at the time of study except for a significant association between lymphocyte culture stimulation by rabbit thymus native DNA and active SLE nephritis. Patients with a membranous antinuclear factor (ANF) pattern had positive skin tests with rabbit thymus native DNA and usually had active disease.

Citing Articles

Cytotoxic responses to alloantigens in systemic lupus erythematosus.

Tsokos G, Balow J J Clin Immunol. 2009; 1(4):208-16.

PMID: 19338096 DOI: 10.1007/BF00915138.

In vitro nucleoside specific immune response by lymphocytes from systemic lupus erythematosus.

Morimoto C, Steinberg A, Schlossman S, Borel Y J Clin Invest. 1983; 71(5):1402-9.

PMID: 6189861 PMC: 437004. DOI: 10.1172/jci110893.

Immunopathologic studies in relapsing polychondritis.

Herman J, Dennis M J Clin Invest. 1973; 52(3):549-58.

PMID: 4265382 PMC: 302292. DOI: 10.1172/JCI107215.

The production of a "universal developer" for the immunological detection of human IgG and its application in immunodiagnostics.

Semenenko F, Kenigsberg R, Cuello A Histochemistry. 1988; 90(4):315-21.

PMID: 3265940 DOI: 10.1007/BF00495976.

Peripheral blood lymphocyte response to phytomitogens in systemic lupus erythematosus.

Foad B, Adams L, Litwin A, Hess E Ann Rheum Dis. 1975; 35(5):407-14.

PMID: 1234408 PMC: 1006572. DOI: 10.1136/ard.35.5.407.

References

Staples P, Gery I, Waksman B . Role of the thymus in tolerance. 3. Tolerance to bovine gamma globulin after direct injection of antigen into the shielded thymus of irradiated rats. J Exp Med. 1966; 124(2):127-39. PMC: 2180475. DOI: 10.1084/jem.124.2.127. View

Mills J . The immunologic significance of antigen induced lymphocyte transformation in vitro. J Immunol. 1966; 97(2):239-47. View

Azoury F, Jones H, DERBES V, Gum O . Intradermal tests and antinuclear factors in systemic lupus erythematosus. A comparative study. Ann Intern Med. 1966; 65(6):1221-8. DOI: 10.7326/0003-4819-65-6-1221. View

Oppenheim J, Wolstencroft R, Gell P . Delayed hypersensitivity in the guinea-pig to a protein-hapten conjugate and its relationship to in vitro transformation of lymph node, spleen, thymus and peripheral blood lymphocytes. Immunology. 1967; 12(1):89-102. PMC: 1409006. View

Patrucco A, Rothfield N, Hirschhorn K . The response of cultured lymphocytes from patients with systemic lupus erythematosus to DNA. Arthritis Rheum. 1967; 10(1):32-7. DOI: 10.1002/art.1780100105. View

Jones H, DERBES V, Gum O, Azoury F . Skin tests with nuclear factors in systemic lupus erythematosus. Arch Dermatol. 1967; 95(6):559-64. View

Block S, Gibbs C, STEVENS M, SHULMAN L . Delayed hypersensitivity in systemic lupus erythematosus. Ann Rheum Dis. 1968; 27(4):311-8. PMC: 1010451. DOI: 10.1136/ard.27.4.311. View

Rees A, KRUEGER R . A new type of deoxyribonucleoprotein isolated from rabbit thymus nuclei. Biochim Biophys Acta. 1968; 161(2):558-60. DOI: 10.1016/0005-2787(68)90133-0. View

Stulbarg M, Schlossman S . The specificity of antigen-induced thymidine-2-14C incorporation into lymph node cells frrom sensitized animals. J Immunol. 1968; 101(4):764-9. View

10.

Teague P, Friou G . Antinuclear antibodies in mice. II. Transmission with spleen cells; inhibition or prevention with thymus or spleen cells. Immunology. 1969; 17(5):665-75. PMC: 1455907. View

11.

Nilsson B . In vitro lymphocyte reactivity to PPD and phytohaemagglutinin in relation to PPD skin reactivity and age. Scand J Respir Dis. 1971; 52(1):39-47. View

12.

Allison A, DENMAN A, Barnes R . Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet. 1971; 2(7716):135-40. DOI: 10.1016/s0140-6736(71)92306-3. View

13.

Playfair J . Strain differences in the immune responses of mice. 3. A raised tolerance threshold in NZB thymus cells. Immunology. 1971; 21(6):1037-43. PMC: 1408265. View

14.

Thomas J, Clements D, Grzybowski S . In vitro lymphocyte responses and skin test reactivity following BCG vaccination. Clin Exp Immunol. 1971; 9(5):611-23. PMC: 1713054. View

15.

Nowell P . Inhibition of human leukocyte mitosis by prednisolone in vitro. Cancer Res. 1961; 21:1518-21. View

16.

TROMOVITCH T, March C . Intradermal tests with autologous white blood cells in chronic discoid lupus erythematosus. systemic lupus erythematosus and control subjects. J Invest Dermatol. 1961; 37:345-50. View

17.

CHIEREGATO G, FALDARINI G . [ON INTRADERMAL INJECTIONS OF AUTO-LEUKOCYTES AND NUCLEIC ACIDS IN LUPUS ERYTHEMATOSUS]. Minerva Dermatol. 1964; 39:108-12. View

18.

Elves M, Gough J, ISRAUELS M . THE PLACE OF THE LYMPHOCYTE IN THE RETICULO-ENDOTHELIAL SYSTEM: A STUDY OF THE IN VITRO EFFECT OF PREDNISOLONE ON LYMPHOCYTES. Acta Haematol. 1964; 32:100-7. DOI: 10.1159/000209561. View

19.

ORES R, Lange K . SKIN TEST FOR THE DIAGNOSIS OF SYSTEMIC LUPUS ERYTHEMATOSUS. Am J Med Sci. 1964; 248:562-6. DOI: 10.1097/00000441-196411000-00007. View

20.

Fardal R, Winkelmann R . HYPERSENSITIVITY TO DEOXYRIBONUCLEIC ACID IN CUTANEOUS DISEASE. Arch Dermatol. 1965; 91:503-11. DOI: 10.1001/archderm.1965.01600110089018. View