A Novel Olefinic Rearrangement. The Enzymic Conversion of Cholesta-7,9-dien-3 -ol into Cholesta-8,14-dien-3 -ol

Overview

Journal Biochem J

Specialty Biochemistry

Date 1972 Sep 1

PMID 4404936

Citations 4

Authors

M Akhtar

C W Freeman

A D Rahimtula

D C Wilton

Affiliations

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Abstract

1. [3alpha-(3)H]Cholesta-7,9-dien-3beta-ol is converted in high yield into cholesterol by a 10000g(av.) supernatant fraction of rat liver homogenate. 2. Incubation of cholesta-7,9-dien-3beta-ol with [4-(3)H]NADPH and rat liver microsomal fractions under anaerobic conditions resulted in (3)H being incorporated into the 14alpha-position of cholest-7-en-3beta-ol. 3. Under anaerobic conditions in the absence of NADPH cholesta-7,9-dien-3beta-ol was isomerized into cholesta-8,14-dien-3beta-ol by rat liver microsomal fractions.

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References

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Watkinson I, Wilton D, Rahimtula A, Akhtar M . The substrate activation in some pyridine nucleotide linked enzymic reactions. The conversion of desmosterol into cholesterol. Eur J Biochem. 1971; 23(1):1-6. DOI: 10.1111/j.1432-1033.1971.tb01583.x. View

Bloxham D, Wilton D, Akhtar M . Studies on the mechanism and regulation of C-4 demethylation in cholesterol biosynthesis. The role of adenosine 3':5'-cyclic monophosphate. Biochem J. 1971; 125(2):625-34. PMC: 1178100. DOI: 10.1042/bj1250625. View