Suppression of Polar Effects of Nonsense Mutations by Ultraviolet Irradiation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1979 Apr 1

PMID 438127

Citations 9

Authors

T J Pollock

E S TESSMAN

I Tessman

Affiliations

Soon will be listed here.

Abstract

Nonsense mutations in capsid genes F and G of phages S13 and phi X174 decreased the expression of genes downstream. These polar effects were suppressed by ultraviolet irradiation of the host before infection. Activities of the downstream genes were restored to between 30 and 95% of their normal levels, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the viral proteins. The effect of ultraviolet irradiation may be to suppress rhodependent termination of transcription. The polarity of one mutation was poorly suppressed by ultraviolet irradiation.

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Suppression of polarity in the gal operon by ultraviolet irradiation.

Fassler J, Tessman I J Bacteriol. 1980; 144(3):1205-7.

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Bacteriophage SPO1 structure and morphogenesis. III. SPO1 proteins and synthesis.

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Control of bacteriophage phi X 174 gene expression by the transcription termination factor rho in vitro.

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Relation between UV suppression of polarity in phi X174 and UV sensitivity of rho mutants.

Fassler J, Tessman I J Virol. 1981; 37(3):955-62.

PMID: 6453239 PMC: 171093. DOI: 10.1128/JVI.37.3.955-962.1981.

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