Immunochemical Procedure for Partial Purification of Newly Synthesized Proteins in Polyoma Virus-infected Mouse Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1974 Mar 1

PMID 4362872

Citations 2

Authors

D Paulin

J PERREAU

F Cuzin

Affiliations

Soon will be listed here.

Abstract

A broad-spectrum antiserum has been obtained against nuclear proteins from normal mouse fibroblasts. Solid phase immunoadsorbants prepared from the serum specifically bind the corresponding antigens. The removal in this way of a significant fraction of host proteins leads to partial purification of viral and virus-induced polypeptides from polyoma virus-infected cell extracts. Four main peptides can be selected, with respective molecular weights of 90,000, 70,000, 46,000, and 41,000.

Citing Articles

Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.

Schaffhausen B, Silver J, BENJAMIN T Proc Natl Acad Sci U S A. 1978; 75(1):79-83.

PMID: 203944 PMC: 411187. DOI: 10.1073/pnas.75.1.79.

Polyoma virus T antigen. I. Synthesis of modified heat-labile T angiten in cells transformed with the ts-a mutant.

Paulin D, Cuzin F J Virol. 1975; 15(2):393-7.

PMID: 163363 PMC: 354465. DOI: 10.1128/JVI.15.2.393-397.1975.

References

Avrameas S, Ternynck T . The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969; 6(1):53-66. DOI: 10.1016/0019-2791(69)90178-5. View

Wicker R, Avrameas S . Localization of virus antigens by enzyme-labelled antibodies. J Gen Virol. 1969; 4(4):465-71. DOI: 10.1099/0022-1317-4-4-465. View

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View

Cuatrecasas P . Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970; 245(12):3059-65. View

Roblin R, Harle E, DULBECCO R . Polyoma virus proteins. 1. Multiple virion components. Virology. 1971; 45(3):555-66. DOI: 10.1016/0042-6822(71)90171-1. View

Fischer H, Sauer G . Identification of virus-induced proteins in cells productively infected with simian virus 40. J Virol. 1972; 9(1):1-9. PMC: 356255. DOI: 10.1128/JVI.9.1.1-9.1972. View

FREARSON P, CRAWFORD L . Polyoma virus basic proteins. J Gen Virol. 1972; 14(2):141-55. DOI: 10.1099/0022-1317-14-2-141. View

Griffith I . Immediate visualization of proteins in dodecyl sulfate-polyacrylamide gels by prestaining with Remazol dyes. Anal Biochem. 1972; 46(2):402-12. DOI: 10.1016/0003-2697(72)90313-2. View

Anderson C, GESTELAND R . Pattern of protein synthesis in monkey cells infected by simian virus 40. J Virol. 1972; 9(5):758-65. PMC: 356371. DOI: 10.1128/JVI.9.5.758-765.1972. View

10.

Walter G, Roblin R, DULBECCO R . Protein synthesis in Simian virus 40-infected monkey cells. Proc Natl Acad Sci U S A. 1972; 69(4):921-4. PMC: 426595. DOI: 10.1073/pnas.69.4.921. View

11.

Sambrook J . Transformation by polyoma virus and simian virus 40. Adv Cancer Res. 1972; 16:141-80. View

12.

Friedmann T, David D . Structural roles of polyoma virus proteins. J Virol. 1972; 10(4):776-82. PMC: 356533. DOI: 10.1128/JVI.10.4.776-782.1972. View

13.

Del Villano B, Defendi V . Characterization of the SV40 T antigen. Virology. 1973; 51(1):34-46. DOI: 10.1016/0042-6822(73)90363-2. View

14.

Studier F . Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973; 79(2):237-48. DOI: 10.1016/0022-2836(73)90003-x. View

15.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

16.

Vogt M, DULBECCO R . Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology. 1962; 16:41-51. DOI: 10.1016/0042-6822(62)90200-3. View

17.

Winocour E . Purification of polyoma virus. Virology. 1963; 19:158-68. DOI: 10.1016/0042-6822(63)90005-9. View