Action of Ribonuclease T1 on 30S Ribosomes of Escherichia Coli and Its Role in Sequence Studies on 16S Ribonucleic Acid

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Dec 1

PMID 4356617

Citations 8

Authors

M Santer

U SANTER

Affiliations

Soon will be listed here.

Abstract

Two large ribonucleic acid (RNA) fragments have been obtained from T1-RNase-treated 30S ribosomes of Escherichia coli. One fragment, about 475 nucleotides long, contains all the unique oligonucleotides found by Fellner and associates in sections of 16S RNA designated P, E, E', and K, and one-half the large oligonucleotides of section A. The other large fragment is about 300 nucleotides long and contains the oligonucleotides found in sections C, C', C''. The isolation of these large fragments seems to confirm the arrangement of sections within 16S RNA. There are also recovered from nuclease-treated ribosomes three small fragments, one (120 nucleotides long) from the 5' end, one (26 nucleotides long) from the 3' OH end of the chain, and another section (66 nucleotides long) from the middle of the 16S RNA chain. Small molecular weight material is also generated by nuclease treatment, and about half this material is derived from a region close to the 3' OH end of the 16S RNA chain. This indicates that the most accessible part of the rRNA of E. coli 30S ribosomes is a region 100 to 150 nucleotides long near the 3' end of the chain. A general scheme is proposed to explain the generation of the various-sized RNA products from the rRNA of the 30S ribosome.

Citing Articles

Long range RNA-RNA interactions in the 30 S ribosomal subunit of E. coli.

SPITNIK-ELSON P, Elson D, Avital S, Abramowitz R Nucleic Acids Res. 1985; 13(13):4719-38.

PMID: 2410855 PMC: 321822. DOI: 10.1093/nar/13.13.4719.

Ribonucleic acid destruction and synthesis during intraperiplasmic growth of Bdellovibrio bacteriovorus.

Hespell R, Miozzari G, RITTENBERG S J Bacteriol. 1975; 123(2):481-91.

PMID: 1097411 PMC: 235752. DOI: 10.1128/jb.123.2.481-491.1975.

Primary sequence of the 16S ribosomal RNA of Escherichia coli.

Ehresmann C, Stiegler P, Mackie G, Zimmermann R, Ebel J, Fellner P Nucleic Acids Res. 1975; 2(2):265-78.

PMID: 1091918 PMC: 342831. DOI: 10.1093/nar/2.2.265.

Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli.

Santer M, Shane S J Bacteriol. 1977; 130(2):900-10.

PMID: 400797 PMC: 235296. DOI: 10.1128/jb.130.2.900-910.1977.

Comparative surfact structure of 16S ribosomal ribonucleic acid of 30S ribosomes of procaryotic cells.

Santer M, Chung S, Harmon G, Estner M, Hendrick J, Hopper J J Bacteriol. 1979; 140(1):131-40.

PMID: 387717 PMC: 216788. DOI: 10.1128/jb.140.1.131-140.1979.

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