Preparation and Properties of Phospholipid Bilayers Containing Rhodopsin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Sep 1

PMID 4341702

Citations 52

Authors

K Hong

W L Hubbell

Affiliations

Soon will be listed here.

Abstract

Purified rhodopsin has been prepared containing less than 1.1 mol of phosphate per mol of protein. The purified rhodopsin has been incorporated into phosphatidylcholine bilayers, and the molecular interactions within the bilayers were investigated by the use of spin-labeled phosphatidylcholines. Rhodopsin appears to inhibit segmental motions of the hydrocarbon chains, an effect similar to that of cholesterol on phospholipid bilayers.

Citing Articles

Tauroursodeoxycholic acid binds to the G-protein site on light activated rhodopsin.

Lobysheva E, Taylor C, Marshall G, Kisselev O Exp Eye Res. 2018; 170:51-57.

PMID: 29454859 PMC: 5983371. DOI: 10.1016/j.exer.2018.02.015.

Rhodopsin gene expression determines rod outer segment size and rod cell resistance to a dominant-negative neurodegeneration mutant.

Price B, Sandoval I, Chan F, Nichols R, Roman-Sanchez R, Wensel T PLoS One. 2012; 7(11):e49889.

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Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa.

Price B, Sandoval I, Chan F, Simons D, Wu S, Wensel T Invest Ophthalmol Vis Sci. 2011; 52(13):9728-36.

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Structure and dynamics of dark-state bovine rhodopsin revealed by chemical cross-linking and high-resolution mass spectrometry.

Jacobsen R, Sale K, Ayson M, Novak P, Hong J, Lane P Protein Sci. 2006; 15(6):1303-17.

PMID: 16731966 PMC: 2242551. DOI: 10.1110/ps.052040406.

Conformational states and dynamics of rhodopsin in micelles and bilayers.

Kusnetzow A, Altenbach C, Hubbell W Biochemistry. 2006; 45(17):5538-50.

PMID: 16634635 PMC: 2739654. DOI: 10.1021/bi060101v.

References

Branton D . Fracture faces of frozen membranes. Proc Natl Acad Sci U S A. 1966; 55(5):1048-56. PMC: 224277. DOI: 10.1073/pnas.55.5.1048. View

Shields J, Dinovo E, Henriksen R, Kimbel Jr R, MILLAR P . The purification and amino acid composition of bovine rhodopsin. Biochim Biophys Acta. 1967; 147(2):238-51. DOI: 10.1016/0005-2795(67)90402-3. View

Heller J . Structure of visual pigments. I. Purification, molecular weight, and composition of bovine visual pigment500. Biochemistry. 1968; 7(8):2906-13. DOI: 10.1021/bi00848a030. View

IRREVERRE F, Stone A, Shichi H, Lewis M . Biochemistry of visual pigments. I. Purification and properties of bovine rhodopsin. J Biol Chem. 1969; 244(4):529-36. View

Gras W, Worthington C . X-ray analysis of retinal photoreceptors. Proc Natl Acad Sci U S A. 1969; 63(2):233-8. PMC: 223551. DOI: 10.1073/pnas.63.2.233. View

Blaurock A, Wilkins M . Structure of frog photoreceptor membranes. Nature. 1969; 223(5209):906-9. DOI: 10.1038/223906a0. View

Blasie J, Worthington C . Planar liquid-like arrangement of photopigment molecules in frog retinal receptor disk membranes. J Mol Biol. 1969; 39(3):417-39. DOI: 10.1016/0022-2836(69)90136-3. View

McConnell H, McFarland B . Physics and chemistry of spin labels. Q Rev Biophys. 1970; 3(1):91-136. DOI: 10.1017/s003358350000442x. View

Tourtellotte M, Branton D, Keith A . Membrane structure: spin labeling and freeze etching of Mycoplasma laidawii. Proc Natl Acad Sci U S A. 1970; 66(3):909-16. PMC: 283137. DOI: 10.1073/pnas.66.3.909. View

10.

Pinto da Silva P, Branton D . Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker. J Cell Biol. 1970; 45(3):598-605. PMC: 2107921. DOI: 10.1083/jcb.45.3.598. View

11.

Rottem S, Hubbell W, Hayflick L, McConnell H . Motion of fatty acid spin labels in the plasma membrane of mycoplasma. Biochim Biophys Acta. 1970; 219(1):104-13. DOI: 10.1016/0005-2736(70)90065-9. View

12.

Deamer D, Leonard R, Tardieu A, Branton D . Lamellar and hexagonal lipid phases visualized by freeze-etching. Biochim Biophys Acta. 1970; 219(1):47-60. DOI: 10.1016/0005-2736(70)90060-x. View

13.

Hubbell W, Metcalfe J, Metcalfe S, McConnell H . The interaction of small molecules with spin-labelled erythrocyte membranes. Biochim Biophys Acta. 1970; 219(2):415-27. DOI: 10.1016/0005-2736(70)90219-1. View

14.

Hubbell W, McConnell H . Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc. 1971; 93(2):314-26. DOI: 10.1021/ja00731a005. View

15.

Zorn M, FUTTERMAN S . Properties of rhodopsin dependent on associated phospholipid. J Biol Chem. 1971; 246(4):881-6. View

16.

Jost P, Libertini L, Hebert V, Griffith O . Lipid spin labels in lecithin multilayers. A study of motion along fatty acid chains. J Mol Biol. 1971; 59(1):77-98. DOI: 10.1016/0022-2836(71)90414-1. View

17.

Seelig J, Hasselbach W . A spin label study of sarcoplasmic vesicles. Eur J Biochem. 1971; 21(1):17-21. DOI: 10.1111/j.1432-1033.1971.tb01434.x. View

18.

Griffith O, Libertini L, Birrell G . The role of lipid spin labels in membrane biophysics. J Phys Chem. 1971; 75(22):3417-25. DOI: 10.1021/j100691a004. View

19.

Shichi H . Circular dichroism of bovine rhodopsin. Photochem Photobiol. 1971; 13(6):499-502. DOI: 10.1111/j.1751-1097.1971.tb06144.x. View

20.

McFarland B, McConnell H . Bent fatty acid chains in lecithin bilayers. Proc Natl Acad Sci U S A. 1971; 68(6):1274-8. PMC: 389170. DOI: 10.1073/pnas.68.6.1274. View