Patterns of Simian Virus 40 DNA Transcription After Acute Infection of Permissive and Nonpermissive Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Jul 1

PMID 4340164

Citations 44

Authors

G Khoury

J C Byrne

M A Martin

Affiliations

Soon will be listed here.

Abstract

Small amounts of fractionated, denatured, (32)P-labeled DNA from SV40 virus were incubated with a large excess of the complementary RNA of SV40 prepared in vitro with Escherichia coli RNA polymerase; the viral DNA strands were separated on hydroxyapatite columns. The RNA present in green monkey cells late in the lytic cycle reacted with 40-42% of the strand complementary to the in vitro complementary RNA (minus strand), and 60-64% of the opposite (plus) strand. "Early lytic" RNA failed to significantly interact with the plus strand, but formed stable duplex molecules with 35-39% of the minus strand. The RNA prepared from mouse embryo cells 24 hr after infection with SV40 combined with 35-38% of the minus strand and 60-62% of the plus strand. In all cases, the same regions of either the plus or minus strand appear to be transcribed in permissive and nonpermissive infections.

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References

Gelb L, Kohne D, Martin M . Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol. 1971; 57(1):129-45. DOI: 10.1016/0022-2836(71)90123-9. View

Westphal H . SV40 DNA strand selection by Escherichia coli RNA polymerase. J Mol Biol. 1970; 50(2):407-20. DOI: 10.1016/0022-2836(70)90201-9. View

Summers W, Siegel R . Transcription of late phage RNA by T7 RNA polymerase. Nature. 1970; 228(5277):1160-2. DOI: 10.1038/2281160a0. View

Sambrook J, Westphal H, Srinivasan P, DULBECCO R . The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968; 60(4):1288-95. PMC: 224916. DOI: 10.1073/pnas.60.4.1288. View

Aloni Y, Winocour E, Sachs L . Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968; 31(3):415-29. DOI: 10.1016/0022-2836(68)90418-x. View

Sauer G . Apparent differences in transcriptional control in cells productively infected and transformed by SV40. Nat New Biol. 1971; 231(22):135-8. DOI: 10.1038/newbio231135a0. View

Earley E, Peralta P, Johnson K . A plaque neutralization method for arboviruses. Proc Soc Exp Biol Med. 1967; 125(3):741-7. DOI: 10.3181/00379727-125-32194. View

Britten R, Kohne D . Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968; 161(3841):529-40. DOI: 10.1126/science.161.3841.529. View

Hudson J, Goldstein D, Weil R . A study on the transcription of the polyoma viral genome. Proc Natl Acad Sci U S A. 1970; 65(1):226-33. PMC: 286214. DOI: 10.1073/pnas.65.1.226. View

10.

Radloff R, BAUER W, Vinograd J . A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967; 57(5):1514-21. PMC: 224502. DOI: 10.1073/pnas.57.5.1514. View

11.

Hirai K, Defendi V . Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of permissive monkey kidney cells. J Virol. 1972; 9(4):705-7. PMC: 356357. DOI: 10.1128/JVI.9.4.705-707.1972. View

12.

Henry P, Black P, Oxman M, Weissman S . Stimulation of DNA synthesis in mouse cell line 3T3 by Simian virus 40. Proc Natl Acad Sci U S A. 1966; 56(4):1170-6. PMC: 220032. DOI: 10.1073/pnas.56.4.1170. View

13.

Chamberlin M, McGrath J, Waskell L . New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970; 228(5268):227-31. DOI: 10.1038/228227a0. View

14.

Martin M, Axelrod D . SV40 gene activity during lytic infection and in a series of SV40 transformed mouse cells. Proc Natl Acad Sci U S A. 1969; 64(4):1203-10. PMC: 223269. DOI: 10.1073/pnas.64.4.1203. View

15.

Todaro G, Green H . Cell growth and the initiation of transformation by SV40. Proc Natl Acad Sci U S A. 1966; 55(2):302-8. PMC: 224140. DOI: 10.1073/pnas.55.2.302. View

16.

Gershon D, Sachs L, Winocour E . The induction of cellular DNA synthesis by simian virus 40 in contact-inhibited and in x-irradiated cells. Proc Natl Acad Sci U S A. 1966; 56(3):918-25. PMC: 219947. DOI: 10.1073/pnas.56.3.918. View

17.

Lindstrom D, DULBECCO R . Strand orientation of simian virus 40 transcription in productively infected cells. Proc Natl Acad Sci U S A. 1972; 69(6):1517-20. PMC: 426739. DOI: 10.1073/pnas.69.6.1517. View