Evidence of Transcription from the Late Region of the Integrated Simian Virus 40 Genome in Transformed Cells: Location of the 5' Ends of Late Transcripts in Cells Abortively Infected and in Cells Transformed by Simian Virus 40

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1983 Jun 1

PMID 6190013

Citations 13

Authors

E May

Affiliations

Soon will be listed here.

Abstract

By means of S1 mapping, we observed that spliced 16S and 19S viral late mRNAs--in addition to early mRNAs--were present in cytoplasmic polyadenylated RNA preparations from simian virus 40-transformed cell lines of rat or mouse origin containing no detectable amount of free viral DNA. The amounts of early and late virus-specific mRNAs in these lines were quantified by hybridization of radioactive cytoplasmic polyadenylated RNA with cloned region-specific restriction fragments. The relative amount of late viral mRNA produced in these transformed cells was found to be of the same order as that produced in simian virus 40-infected, nonpermissive baby mouse kidney cells. Moreover, by using the S1 nuclease protection method, we compared the 5' ends of late mRNAs produced (i) in transformed cells, (ii) in abortively infected mouse cells, and (iii) in the late phase of the lytic cycle. The 5' ends of late mRNAs both in abortively infected and in transformed cells were less heterogeneous than the 5' ends of late mRNAs produced during the lytic cycle; however, they were a subset of the 5' ends of late transcripts produced in the lytic cycle.

Citing Articles

SV40 lymphomagenesis in Syrian golden hamsters.

McNees A, Vilchez R, Heard T, Sroller V, Wong C, Herron A Virology. 2008; 384(1):114-24.

PMID: 19038412 PMC: 3648995. DOI: 10.1016/j.virol.2008.10.035.

Conversion through homologous recombination of the gene encoding Simian virus 40 115,000-molecular-weight super T antigen to a gene encoding a normal-size large T antigen variant.

May P, Resche-Rigon M, Borde J, Breugnot C, May E Mol Cell Biol. 1984; 4(6):1141-51.

PMID: 6330531 PMC: 368883. DOI: 10.1128/mcb.4.6.1141-1151.1984.

Simian virus 40 late promoter region able to initiate simian virus 40 early gene transcription in the absence of the simian virus 40 origin sequence.

May P, Moreau P, May E J Virol. 1984; 50(1):163-73.

PMID: 6321788 PMC: 255596. DOI: 10.1128/JVI.50.1.163-173.1984.

Mapping of the late promoter of simian virus 40.

Hartzell S, Byrne B, Subramanian K Proc Natl Acad Sci U S A. 1984; 81(1):23-7.

PMID: 6320166 PMC: 344602. DOI: 10.1073/pnas.81.1.23.

The sequence motifs that are involved in SV40 enhancer function also control SV40 late promoter activity.

May E, Omilli F, Zenke M, Chambon P Nucleic Acids Res. 1987; 15(6):2445-61.

PMID: 3031598 PMC: 340662. DOI: 10.1093/nar/15.6.2445.

References

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

Reeder R . The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis. Cell. 1979; 18(2):485-99. DOI: 10.1016/0092-8674(79)90066-7. View

Baker C, Herisse J, Courtois G, Galibert F, Ziff E . Messenger RNA for the Ad2 DNA binding protein: DNA sequences encoding the first leader and heterogenity at the mRNA 5' end. Cell. 1979; 18(2):569-80. DOI: 10.1016/0092-8674(79)90073-4. View

Weaver R, Weissmann C . Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res. 1979; 7(5):1175-93. PMC: 342295. DOI: 10.1093/nar/7.5.1175. View

Chumakov P . Transcription of the viral genome in cell lines transformed by simian virus 40. I. Mapping of virus-specific nuclear RNAs. Nucleic Acids Res. 1980; 8(1):111-25. PMC: 327246. DOI: 10.1093/nar/8.1.111. View

Daya-Grosjean L, Lasne C, Nardeux P, CHOUROULINKOV I, Monier R . Oncogenic transformation of rat lung epitheloid cells by SV 40 DNA and restriction enzyme fragments. Arch Virol. 1979; 62(2):87-100. DOI: 10.1007/BF01318062. View

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

Hentschel C, Irminger J, Bucher P, Birnstiel M . Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences. Nature. 1980; 285(5761):147-51. DOI: 10.1038/285147a0. View

SCHWYZER M, Weil R, FRANK G, Zuber H . Amino acid sequence analysis of fragments generated by partial proteolysis from large simian virus 40 tumor antigen. J Biol Chem. 1980; 255(12):5627-34. View

10.

Handa H, Sharp P . Expression of early and late simian virus 40 transcripts in the absence of protein synthesis. J Virol. 1980; 34(3):592-7. PMC: 288746. DOI: 10.1128/JVI.34.3.592-597.1980. View

11.

May P, Kress M, Lange M, May E . New genetic information expressed in SV40-transformed cells: characterization of the 55K proteins and evidence for unusual SV40 mRNAs. Cold Spring Harb Symp Quant Biol. 1980; 44 Pt 1,:189-200. DOI: 10.1101/sqb.1980.044.01.022. View

12.

Wilson M, Nevins J, Blanchard J, Ginsberg H, Darnell Jr J . Metabolism of mRNA from the transforming region of adenovirus 2. Cold Spring Harb Symp Quant Biol. 1980; 44 Pt 1,:447-55. DOI: 10.1101/sqb.1980.044.01.048. View

13.

Gannon F, Jeltsch J, Perrin F . A detailed comparison of the 5'-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA. Nucleic Acids Res. 1980; 8(19):4405-21. PMC: 324248. DOI: 10.1093/nar/8.19.4405. View

14.

Contreras R, Fiers W . Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CVI cells; evidence for multiple promoters of SV40 late transcription. Nucleic Acids Res. 1981; 9(2):215-36. PMC: 326688. DOI: 10.1093/nar/9.2.215. View

15.

May E, Kress M, Daya-Grosjean L, Monier R, May P . Mapping of the viral mRNA encoding a super-T antigen of 115,000 daltons expressed in simian virus 40-transformed rat cell lines. J Virol. 1981; 37(1):24-35. PMC: 170978. DOI: 10.1128/JVI.37.1.24-35.1981. View

16.

Hu S, Manley J . DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2. Proc Natl Acad Sci U S A. 1981; 78(2):820-4. PMC: 319894. DOI: 10.1073/pnas.78.2.820. View

17.

Lange M, May E, May P . Ability of nonpermissive mouse cells to express a simian virus 40 late function(s). J Virol. 1981; 38(3):940-51. PMC: 171232. DOI: 10.1128/JVI.38.3.940-951.1981. View

18.

Gidoni D, Kahana C, Canaani D, Groner Y . Specific in vitro initiation of transcription of simian virus 40 early and late genes occurs at the various cap nucleotides including cytidine. Proc Natl Acad Sci U S A. 1981; 78(4):2174-8. PMC: 319306. DOI: 10.1073/pnas.78.4.2174. View

19.

Dretzen G, BELLARD M, Sassone-Corsi P, Chambon P . A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981; 112(2):295-8. DOI: 10.1016/0003-2697(81)90296-7. View

20.

Breathnach R, Chambon P . Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981; 50:349-83. DOI: 10.1146/annurev.bi.50.070181.002025. View