In Vitro Translation of Cardiovirus Ribonucleic Acid by Mammalian Cell-free Extracts

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1972 Apr 1

PMID 4336562

Citations 20

Authors

K L Eggen

A J Shatkin

Affiliations

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Abstract

Cell-free extracts prepared from Ehrlich ascites and mouse L cells synthesize viral proteins in response to encephalomyocarditis virus, mouse Elberfeld virus, and mengovirus ribonucleic acid. Although HeLa cell extracts are inactive, their ribosomes are functional in the presence of heterologous supernatant fractions. Synthesis depends upon the addition of adenosine triphosphate, guanosine triphosphate, an energy-generating system, and 4 mm Mg(2+). Initiation is completed during the first 10 to 20 min of incubation, but chain elongation continues for 1 hr or more. The products are of higher molecular weight than virion structural proteins and resemble polypeptides formed in virus-infected cells during a short pulse. Tryptic peptides of virion proteins and in vitro products are similar for all three cardioviruses.

Citing Articles

Inhibition of Protein Synthesis in Cell-Free Systems from Interferon-Treated, Infected Cells: Further Characterization and Effect of Formylmethionyl-tRNA(F).

Kerr I, Friedman R, Brown R, Ball L, Brown J J Virol. 1974; 13(1):9-21.

PMID: 16789138 PMC: 355252. DOI: 10.1128/JVI.13.1.9-21.1974.

Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.

Sonenberg N, Guertin D, Lee K Mol Cell Biol. 1982; 2(12):1633-8.

PMID: 14582204 PMC: 369971. DOI: 10.1128/mcb.2.12.1633-1638.1982.

Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.

Lee K, Sonenberg N Proc Natl Acad Sci U S A. 1982; 79(11):3447-51.

PMID: 6954488 PMC: 346437. DOI: 10.1073/pnas.79.11.3447.

Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo.

Davies J, Kaesberg P J Virol. 1973; 12(6):1434-41.

PMID: 4586778 PMC: 356785. DOI: 10.1128/JVI.12.6.1434-1441.1973.

Cell-free synthesis of rat growth hormone.

Bancroft F, Wu G, Zubay G Proc Natl Acad Sci U S A. 1973; 70(12):3646-9.

PMID: 4519652 PMC: 427298. DOI: 10.1073/pnas.70.12.3646.

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