Quantitative Isolation of Total Glycosphingolipids from Animal Cells

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 1971 Mar 1

PMID 4324310

Citations 97

Authors

T Saito

S I HAKOMORI

Affiliations

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Abstract

The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia-silica gel (Florisil) column, and (c) deacetylation of glycolipid in chloroform-methanol-sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.

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