Characterization of Bluetongue Virus Ribonucleic Acid

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1970 Jan 1

PMID 4315158

Citations 47

Authors

D W Verwoerd

H Louw

R A Oellermann

Affiliations

Soon will be listed here.

Abstract

An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.

Citing Articles

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PMID: 39599497 PMC: 11597718. DOI: 10.3390/pathogens13110944.

The Combined Expression of the Nonstructural Protein NS1 and the N-Terminal Half of NS2 (NS2) by ChAdOx1 and MVA Confers Protection against Clinical Disease in Sheep upon Bluetongue Virus Challenge.

Utrilla-Trigo S, Jimenez-Cabello L, Calvo-Pinilla E, Marin-Lopez A, Lorenzo G, Sanchez-Cordon P J Virol. 2021; 96(3):e0161421.

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Differential Localization of Structural and Non-Structural Proteins during the Bluetongue Virus Replication Cycle.

Mohl B, Kerviel A, Labadie T, Matsuo E, Roy P Viruses. 2020; 12(3).

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PCR-based reverse genetics strategy for bluetongue virus recovery.

Xu Q, Ge J, Li M, Sun E, Zhou Y, Guo Y Virol J. 2019; 16(1):151.

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Hsp90 Chaperones Bluetongue Virus Proteins and Prevents Proteasomal Degradation.

Mohl B, Roy P J Virol. 2019; 93(20).

PMID: 31375577 PMC: 6798104. DOI: 10.1128/JVI.00898-19.

References

Bellamy A, Shapiro L, August J, Joklik W . Studies on reovirus RNA. I. Characterization of reovirus genome RNA. J Mol Biol. 1967; 29(1):1-17. DOI: 10.1016/0022-2836(67)90177-5. View

Howell P, Verwoerd D, Oellermann R . Plaque formation by bluetongue virus. Onderstepoort J Vet Res. 1967; 34(2):317-32. View

Shatkin A, Sipe J . Single-stranded, adenine-rich RNA from purified reoviruses. Proc Natl Acad Sci U S A. 1968; 59(1):246-53. PMC: 286028. DOI: 10.1073/pnas.59.1.246. View

Shatkin A, Sipe J, Loh P . Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J Virol. 1968; 2(10):986-91. PMC: 375428. DOI: 10.1128/JVI.2.10.986-991.1968. View

Verwoerd D . Purification and characterization of bluetongue virus. Virology. 1969; 38(2):203-12. DOI: 10.1016/0042-6822(69)90361-4. View

Watanabe Y, GRAHAM A . Structural units of reovirus ribonucleic acid and their possible functional significance. J Virol. 1967; 1(4):665-77. PMC: 375333. DOI: 10.1128/JVI.1.4.665-677.1967. View

McEwen C . Tables for estimating sedimentation through linear concentration gradients of sucrose solution. Anal Biochem. 1967; 20(1):114-49. DOI: 10.1016/0003-2697(67)90271-0. View

Watanabe Y, Millward S, GRAHAM A . Regulation of transcription of the Reovirus genome. J Mol Biol. 1968; 36(1):107-23. DOI: 10.1016/0022-2836(68)90223-4. View

Scherrer K, Darnell J . Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962; 7:486-90. DOI: 10.1016/0006-291x(62)90341-8. View

10.

Studier F . SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965; 11:373-90. DOI: 10.1016/s0022-2836(65)80064-x. View

11.

Katz L, Penman S . The solvent denaturation of double-stranded RNA from poliovirus infected HeLa cells. Biochem Biophys Res Commun. 1966; 23(4):557-60. DOI: 10.1016/0006-291x(66)90765-0. View

12.

Loening U . The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967; 102(1):251-7. PMC: 1270235. DOI: 10.1042/bj1020251. View

13.

Vasquez C, KLEINSCHMIDT A . Electron microscopy of RNA strands released from individual Reovirus particles. J Mol Biol. 1968; 34(1):137-47. DOI: 10.1016/0022-2836(68)90240-4. View