The Incorporation of Cytidine 5'-monophosphate and Other Ribonucleotides by an Enzyme Preparation from Rat-liver Microsomes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1967 Jan 1

PMID 4291558

Citations 2

Authors

M G Page

G R Haynes

H G KLEMPERER

Affiliations

Soon will be listed here.

Abstract

1. An enzyme preparation from rat-liver microsomes incorporated all four ribonucleotides from the corresponding triphosphates into ribosomal RNA. The reaction was Mn(2+)-dependent, but UMP incorporation also occurred in the presence of Mg(2+). 2. The incorporation of any one ribonucleotide was inhibited by the presence of the other three ribonucleoside triphosphates and by denatured DNA. 3. The product of the reaction consisted of short chains of homopolymer attached to the primer ribosomal RNA. 4. ;Soluble' RNA, synthetic polyribonucleotides, and oligoribonucleotides were also effective primers for CMP incorporation. 5. When phosphodiesterase-treated ;soluble' RNA was the primer, CMP was incorporated into positions usually occupied by the normal terminal trinucleotide sequence of intact ;soluble' RNA, but the enzyme did not synthesize a specific terminal sequence consisting of a defined number of CMP residues.

Citing Articles

Evidence for defective transfer ribonucleic acid in polymyopathic hamsters and its inhibitory effect on protein synthesis.

Bester A, Gevers W Biochem J. 1973; 132(2):203-14.

PMID: 4725037 PMC: 1177579. DOI: 10.1042/bj1320203.

Altered specificity of transfer-ribonucleic acid nucleotidyltransferase in the presence of manganese.

KLEMPERER H, Haynes G Biochem J. 1967; 104(2):537-44.

PMID: 4292775 PMC: 1270617. DOI: 10.1042/bj1040537.

References

Chakravorty A, Biswas B . Nonterminal incorporation of guanosine monophosphate from guanosine triphosphate by an enzyme system from spinach chloroplasts. J Biol Chem. 1965; 240(11):4406-13. View

Edmonds M . A cytidine triphosphate polymerase from thymus nuclei. 1. Purification and properties of the enzyme and its polynucleotide primer. J Biol Chem. 1965; 240(12):4621-8. View

Wykes J, SMELLIE R . The synthesis of polyribonucleotides by cytoplasmic enzymes. Biochem J. 1966; 99(2):347-55. PMC: 1265003. DOI: 10.1042/bj0990347. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

KUBY S, NODA L, LARDY H . Adenosinetriphosphate-creatine transphosphorylase. I. Isolation of the crystalline enzyme from rabbit muscle. J Biol Chem. 1954; 209(1):191-201. View

Canellakis E . Incorporation of radioactive uridine-5'-monophosphate into ribonucleic acid by soluble mammalian enzymes. Biochim Biophys Acta. 1957; 23(1):217-8. DOI: 10.1016/0006-3002(57)90318-9. View

Kelly C, Layne S . Bacteria found in the air over Canada and the American Arctic. Can J Microbiol. 1957; 3(3):447-55. DOI: 10.1139/m57-047. View

Koerner J, SINSHEIMER R . A deoxyribonuclease from calf spleen. II. Mode of action. J Biol Chem. 1957; 228(2):1049-62. View

Hecht L, Zamecnik P, Stephenson M, Scott J . Nucleoside tri-phosphates as precursors of ribonucleic acid end groups in a mammalian system. J Biol Chem. 1958; 233(4):954-63. View

10.

BURDON R, SMELLIE R . The incorporation of uridine 5'-triphosphate into ribonucleic acid by enzyme fractions from Ehrlich ascites carcinoma cells. Biochim Biophys Acta. 1961; 51:153-62. DOI: 10.1016/0006-3002(61)91026-5. View

11.

Canellakis E, Herbert E . Studies on s-RNA synthesis. II. Assay and method of purification of s-RNA alpha, beta and gamma. Biochim Biophys Acta. 1960; 45:133-8. DOI: 10.1016/0006-3002(60)91433-5. View

12.

Hurwitz J, BRESLER A . The incorporation of ribonucleotides into ribonucleic acid. J Biol Chem. 1961; 236:542-8. View

13.

KAMMEN H, KLEMPERER H, Canellakis E . The binding of nucleoside di- and triphosphates by a rat-liver component. Biochim Biophys Acta. 1961; 51:175-7. DOI: 10.1016/0006-3002(61)91032-0. View

14.

Edmonds M, Abrams R . Nature of a polynucleotide required for polyribonucleotide formation from adenosine triphosphate with an enzyme from thymus nuclei. J Biol Chem. 1962; 237:2636-42. View

15.

Josse J, Kornberg A . Glucosylation of deoxyribonucleic acid. III. alpha- and beta-Glucosyl transferases from T4-infected Escherichia coli. J Biol Chem. 1962; 237:1968-76. View

16.

August J, ORTIZ P, Hurwitz J . Ribonucleic acid-dependent ribonucleotide incorporation. I. Purification and properties of the enzyme. J Biol Chem. 1962; 237:3786-93. View

17.

VENKATARAMAN P, Mahler H . Incorporation of adenylic acid into ribonucleic acid and synthetic polynucleotides. J Biol Chem. 1963; 238:1058-67. View

18.

BURDON R . The uptake of ribonucleotide 5'-trephosphates by nuclear ribosomes. Biochem Biophys Res Commun. 1963; 11:472-6. DOI: 10.1016/0006-291x(63)90095-0. View

19.

KLEMPERER H . Manganous ion-dependent incorporation of adenosine-5'-phosphate into ribonucleic acid by an enzyme from rat liver. Biochim Biophys Acta. 1963; 72:416-20. View

20.

Baltimore D, Franklin R . A NEW RIBONUCLEIC ACID POLYMERASE APPEARING AFTER MENGOVIRUS INFECTION OF L-CELLS. J Biol Chem. 1963; 238:3395-400. View