The Isolation and Selected Properties of Blood Monocytes

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1966 Jan 1

PMID 4285446

Citations 113

Authors

W E BENNETT

Z A COHN

Affiliations

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Abstract

A technique is described for the quantitative recovery of monocytes from horse blood by means of flotation on dense albumin solutions. Monocytes are concentrated in a surface pellicle along with a few lymphocytes which are then removed when the monocytes adhere to a glass surface. The in vitro cultivation of homogeneous populations of monocytes results in an increase in (a) cell size, (b) number of mitochondria, and (c) phase-dense granules of the centrosphere. The phase-dense granules are osmiophilic and acid phosphatase positive. Quantitative biochemical analysis during cultivation have revealed increased levels of cytochrome oxidase, acid phosphatase, arylsulfatase, and BPN hydrolase. In addition, glucose utilization and lactic acid production are stimulated under the same conditions. The uptake of both bacteria and colloidal gold is stimulated during in vitro cultivation. The phagocytic activity of cultured monocytes may be enhanced by a purified bacterial lipopolysaccharide. These data are consistant with the in vitro maturation of monocytes to macrophages, a cell with greater metabolic and functional potentional.

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References

COHN Z, Benson B . The in vitro differentiation of mononuclear phagocytes. 3. The reversibility of granule and hydrolytic enzyme formation and the turnover of granule constituents. J Exp Med. 1965; 122(3):455-66. PMC: 2138068. DOI: 10.1084/jem.122.3.455. View

Seligman A, CHAUNCEY H, NACHLAS M, MANHEIMER L, Ravin H . The colorimetric determination of phosphatases in human serum. J Biol Chem. 1951; 190(1):7-15. View

WEISS L, Fawcett D . Cytochemical observations on chicken monocytes macrophages and giant cells in tissue culture. J Histochem Cytochem. 1953; 1(1):47-65. DOI: 10.1177/1.1.47. View

Ravin H, Bernstein P, Seligman A . A colorimetric micromethod for the estimation of chymotrypsin activity. J Biol Chem. 1954; 208(1):1-15. View

Nielsen S, Lehninger A . Phosphorylation coupled to the oxidation of ferrocytochrome c. J Biol Chem. 1955; 215(2):555-70. View

BRUNS F, HORN H . [Quantitative determination of L-(+)lactic acid with lactic dehydrogenase]. Biochim Biophys Acta. 1956; 21(2):378-80. DOI: 10.1016/0006-3002(56)90023-3. View

Bonting S, Jones M . Determination of microgram quantities of deoxyribonucleic acid and protein in tissues grown in vitro. Arch Biochem Biophys. 1957; 66(2):340-53. DOI: 10.1016/s0003-9861(57)80009-5. View

Rowley D . Antibacterial systems of serum in relation to nonspecific immunity to infection. Bacteriol Rev. 1960; 24(1):106-14. PMC: 441040. DOI: 10.1128/br.24.1.106-114.1960. View

Rabinowitz Y, SCHREK R . Studies of cell source of macrophages from human blood in slide chambers. Proc Soc Exp Biol Med. 1962; 110:429-31. DOI: 10.3181/00379727-110-27539. View

10.

Tanaka K, Valentine W, FREDRICKS R . Human leucocyte arylsulphatase activity. Br J Haematol. 1962; 8:86-92. DOI: 10.1111/j.1365-2141.1962.tb06498.x. View

11.

Thorbecke G, Benacerraf B . The reticulo-endothelial system and immunological phenomena. Prog Allergy. 1962; 6:559-98. View

12.

Oren R, Farnham A, Saito K, MILOFSKY E, Karnovsky M . Metabolic patterns in three types of phagocytizing cells. J Cell Biol. 1963; 17:487-501. PMC: 2106210. DOI: 10.1083/jcb.17.3.487. View

13.

SPECTOR W, Willoughby D . The inflammatory response. Bacteriol Rev. 1963; 27:117-54. PMC: 441175. DOI: 10.1128/br.27.2.117-154.1963. View

14.

WULFF H . Histochemical studies of leukocytes from an inflammatory exudate. II. Succinic dehydrogenase, mitochondrial alpha-glycerophosphate dehydrogenase and di- and triphosphopyridine nucleotide diaphorases. Acta Haematol. 1963; 29:208-17. DOI: 10.1159/000207346. View

15.

Jee W, NOLAN P . ORIGIN OF OSTEOCLASTS FROM THE FUSION OF PHAGOCYTES. Nature. 1963; 200:225-6. DOI: 10.1038/200225a0. View

16.

Dannenberg Jr A, BENNETT W . HYDROLYTIC ENZYMES OF RABBIT MONONUCLEAR EXUDATE CELLS. I. QUANTITATIVE ASSAY AND PROPERTIES OF CERTAIN PROTEASES, NON-SPECIFIC ESTERASES, AND LIPASES OF MONONUCLEAR AND POLYMORPHONUCLEAR CELLS AND ERYTHROCYTES. J Cell Biol. 1964; 21:1-13. PMC: 2106429. DOI: 10.1083/jcb.21.1.1. View

17.

Rabinowitz Y . SEPARATION OF LYMPHOCYTES, POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES ON GLASS COLUMNS, INCLUDING TISSUE CULTURE OBSERVATIONS. Blood. 1964; 23:811-28. View

18.

COHN Z, Benson B . THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY. J Exp Med. 1965; 121:153-70. PMC: 2137972. DOI: 10.1084/jem.121.1.153. View

19.

COHN Z, Benson B . THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. II. THE INFLUENCE OF SERUM ON GRANULE FORMATION, HYDROLASE PRODUCTION, AND PINOCYTOSIS. J Exp Med. 1965; 121:835-48. PMC: 2138006. DOI: 10.1084/jem.121.5.835. View

20.

Martin A, NEUFELD H, Lucas F, Stotz E . Characterization of uterine peroxidase. J Biol Chem. 1958; 233(1):206-8. View