Article: Transduction of tetracycline resistance in Staphylococcus epidermidis

Fourteen Staphylococcus epidermidis strains were biotyped according to the scheme of Baird-Parker and tested for their sensitivities to S. epidermidis typing phages. Tetracycline-susceptible strains were tested for the capacity to be transduced to resistance. Five strains, all of biotype 1, were lysed by one or more phages. Five of eight biotype 1 strains were transduced to tetracycline resistance with rates of 10(-8) to 10(-5). Three strains of other biotypes were not transduced, nor was PS 73, an unusual coagulase-negative strain. The transducing phage phi367 was isolated from the lysogenic tetracycline-resistant donor strain. The phage morphologically resembles serological group B S. aureus phages. Restriction and modification were indicated from the efficiency of plating on phage-sensitive strains.

Citing Articles

Transfer of plasmid DNA to clinical coagulase-negative staphylococcal pathogens by using a unique bacteriophage.

Winstel V, Kuhner P, Krismer B, Peschel A, Rohde H Appl Environ Microbiol. 2015; 81(7):2481-8.

PMID: 25616805 PMC: 4357934. DOI: 10.1128/AEM.04190-14.

Plasmid for tetracycline resistance in Staphylococcus epidermidis.

Minshew B, ROSENBLUM E Antimicrob Agents Chemother. 1973; 3(5):568-74.

PMID: 4790612 PMC: 444459. DOI: 10.1128/AAC.3.5.568.

Virulence factors of biotypes of Staphylococcus epidermidis from clinical sources.

Males B, Rogers Jr W, Parisi J J Clin Microbiol. 1975; 1(3):256-61.

PMID: 1176603 PMC: 275050. DOI: 10.1128/jcm.1.3.256-261.1975.

Development of a bacteriophage-typing set for Staphylococcus epidermidis.

Skahan J, Parisi J J Clin Microbiol. 1977; 6(1):16-8.

PMID: 142095 PMC: 274689. DOI: 10.1128/jcm.6.1.16-18.1977.

Evaluation of a new bacteriophage set for typing of Staphylococcus epidermidis strains.

Heczko P, Pulverer G, Kasprowicz A, Klein A J Clin Microbiol. 1977; 5(6):573-7.

PMID: 142094 PMC: 274658. DOI: 10.1128/jcm.5.6.573-577.1977.

References

1.

Verhoef J, WINKLER K, van Boven C . Characters of phages from coagulase-negative staphylococci. J Med Microbiol. 1971; 4(4):413-24. DOI: 10.1099/00222615-4-4-413. View

2.

Verhoef J, WINKLER K, van Boven C . Lysogeny in coagulase-negative staphylococci. J Med Microbiol. 1971; 4(4):405-12. DOI: 10.1099/00222615-4-4-405. View

3.

Holt R . The classification of staphylococci from colonized ventriculo-atrial shunts. J Clin Pathol. 1969; 22(4):475-82. PMC: 474215. DOI: 10.1136/jcp.22.4.475. View

4.

ROSENBLUM E, TYRONE S . SEROLOGY, DENSITY, AND MORPHOLOGY OF STAPHYLOCOCCAL PHAGES. J Bacteriol. 1964; 88:1737-42. PMC: 277480. DOI: 10.1128/jb.88.6.1737-1742.1964. View

5.

BAIRD-PARKER A . A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963; 30:409-27. DOI: 10.1099/00221287-30-3-409. View

6.

BAIRD-PARKER A . Staphylococci and their classification. Ann N Y Acad Sci. 1965; 128(1):4-25. DOI: 10.1111/j.1749-6632.1965.tb11626.x. View

7.

SWANSTROM M, Adams M . Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951; 78(2):372-5. DOI: 10.3181/00379727-78-19076. View

8.

Verhoef J, van Boven C, Holtrigter B . Restriction and modification of phages in coagulase-negative staphylococci. Antonie Van Leeuwenhoek. 1971; 37(2):256-7. DOI: 10.1007/BF02218490. View

9.

Yu L, Baldwin J . Intraspecific transduction in Staphylococcus epidermidis and interspecific transduction between Staphylococcus aureus and Staphylococcus epidermidis. Can J Microbiol. 1971; 17(6):767-73. DOI: 10.1139/m71-122. View

10.

Collins A, McDonald S . Transduction of tetracycline resistance in Staphylococci. J Pathol Bacteriol. 1962; 83:399-404. DOI: 10.1002/path.1700830209. View

11.

COMTOIS R . Lysogeny in a coagulase-negative Staphylococcus. Can J Microbiol. 1962; 8:201-11. DOI: 10.1139/m62-026. View

12.

van Boven C, Verhoef J, WINKLER K . Bacteriophage typing of coagulase-negative staphylococci. Antonie Van Leeuwenhoek. 1969; 35(2):232. DOI: 10.1007/BF02219134. View

Transduction of Tetracycline Resistance in Staphylococcus Epidermidis