Transcriptional Control in the L-arabinose Operon of Escherichia Coli B-r

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Apr 1

PMID 4206866

Citations 7

Authors

P P Cleary

E ENGLESBERG

Affiliations

Soon will be listed here.

Abstract

The structural genes involved in l-arabinose metabolism are regulated by the protein product of the araC gene. This protein functions as both an activator and repressor of enzyme synthesis in this gene complex. Using lambdah80dara deoxyribonucleic acid in hybridization studies, we have shown that the ara operon, including structural genes araB, araA, and araD, is transcribed in the direction araB to araD and that initiation of transcription of these genes requires an active araC gene. The half-life of this message, approximately 3 min at 30 C, is the same in the presence or absence of the araC protein in the activator state. However, an unexplained 2-min lag in decay of ara messenger ribonucleic acid that does not occur in decay of lac messenger ribonucleic acid is observed. This lag period requires activated araC protein.

Citing Articles

FREQ-Seq2: a method for precise high-throughput combinatorial quantification of allele frequencies.

Zhao R, Lukacsovich T, Gaut R, Emerson J G3 (Bethesda). 2023; 13(10).

PMID: 37494033 PMC: 10542570. DOI: 10.1093/g3journal/jkad162.

The construction in vitro of derivatives of bacteriophage lambda carrying the amidase genes of Pseudomonas aeruginosa.

Drew R, Clarke P, Brammar W Mol Gen Genet. 1980; 177(2):311-20.

PMID: 6245342 DOI: 10.1007/BF00267444.

Transcriptional control of the isoleucine-valine messenger RNA's in E. coli K-12.

Childs G, Freundlich M Mol Gen Genet. 1975; 138(3):257-68.

PMID: 1102933 DOI: 10.1007/BF00269352.

Recalibrated linkage map of Escherichia coli K-12.

Bachmann B, Low K, Taylor A Bacteriol Rev. 1976; 40(1):116-67.

PMID: 773363 PMC: 413944. DOI: 10.1128/br.40.1.116-167.1976.

The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r.

Bass R, Heffernan L, Sweadner K, ENGLESBERG E Arch Microbiol. 1976; 110(1):135-43.

PMID: 189718 DOI: 10.1007/BF00416978.

References

ENGLESBERG E, Irr J, Power J, Lee N . Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol. 1965; 90(4):946-57. PMC: 315760. DOI: 10.1128/jb.90.4.946-957.1965. View

KESSLER D, ENGLESBERG E . Arabinose-leucine deletion mutants of Escherichia coli B-r. J Bacteriol. 1969; 98(3):1159-69. PMC: 315309. DOI: 10.1128/jb.98.3.1159-1169.1969. View

ENGLESBERG E, Squires C, Meronk Jr F . The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. Proc Natl Acad Sci U S A. 1969; 62(4):1100-7. PMC: 223620. DOI: 10.1073/pnas.62.4.1100. View

Morse D, Yanofsky C . A transcription-initiating mutation within a structural gene of the tryptophan operon. J Mol Biol. 1969; 41(3):317-28. DOI: 10.1016/0022-2836(69)90278-2. View

Studier F, Maizel Jr J . T7-directed protein synthesis. Virology. 1969; 39(3):575-86. DOI: 10.1016/0042-6822(69)90105-6. View

Taylor A . Current linkage map of Escherichia coli. Bacteriol Rev. 1970; 34(2):155-75. PMC: 408314. DOI: 10.1128/br.34.2.155-175.1970. View

Rose J, Mosteller R, Yanofsky C . Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970; 51(3):541-50. DOI: 10.1016/0022-2836(70)90007-0. View

Varmus H, Perlman R, Pastan I . Regulation of lac transcription in antibiotic-treated E. coli. Nat New Biol. 1971; 230(10):41-4. DOI: 10.1038/newbio230041a0. View

Beverin S, Sheppard D, Park S . D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC. J Bacteriol. 1971; 107(1):79-86. PMC: 246888. DOI: 10.1128/jb.107.1.79-86.1971. View

10.

Zubay G, Gielow L, ENGLESBERG E . Cell-free studies on the regulation of the arabinose operon. Nat New Biol. 1971; 233(40):164-5. DOI: 10.1038/newbio233164a0. View

11.

GREENBLATT J, Schleif R . Arabinose C protein: regulation of the arabinose operon in vitro. Nat New Biol. 1971; 233(40):166-70. DOI: 10.1038/newbio233166a0. View

12.

Wilcox G, Singer J, Heffernan L . Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r. J Bacteriol. 1971; 108(1):1-4. PMC: 247024. DOI: 10.1128/jb.108.1.1-4.1971. View

13.

Wilcox G, Clemetson K, Santi D, ENGLESBERG E . Purification of the araC protein. Proc Natl Acad Sci U S A. 1971; 68(9):2145-8. PMC: 389371. DOI: 10.1073/pnas.68.9.2145. View

14.

Marrs B, Yanofsky C . Host and bacteriophage specific messenger RNA degradation in T7-infected Escherichia coli. Nat New Biol. 1971; 234(49):168-70. DOI: 10.1038/newbio234168a0. View

15.

Gielow L, Largen M, ENGLESBERG E . Initiator constitutive mutants of the L-arabinose operon (OIBAD) of Escherichia coli B/r. Genetics. 1971; 69(3):289-302. PMC: 1212706. DOI: 10.1093/genetics/69.3.289. View

16.

Schleif R . L-arabinose operon messenger of Escherichia coli. Its inducibility and translation efficiency relative to lactose operon messenger. J Mol Biol. 1971; 61(1):275-9. DOI: 10.1016/0022-2836(71)90226-9. View

17.

Wu A, Ghosh S, Echols H, Spiegelman W . Repression by the cI protein of phage lambda: in vitro inhibition of RNA synthesis. J Mol Biol. 1972; 67(3):407-21. DOI: 10.1016/0022-2836(72)90459-7. View

18.

Yang H, Zubay G . Synthesis of the arabinose operon regulator protein in a cell-free system. Mol Gen Genet. 1973; 122(2):131-6. DOI: 10.1007/BF00435186. View

19.

ENGLESBERG E . Enzymatic characterization of 17 L-arabinose negative mutants of Escherichia coli. J Bacteriol. 1961; 81:996-1006. PMC: 314771. DOI: 10.1128/jb.81.6.996-1006.1961. View

20.

ENGLESBERG E, Anderson R, Weinberg R, Lee N, HOFFEE P, HUTTENHAUER G . L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J Bacteriol. 1962; 84:137-46. PMC: 277779. DOI: 10.1128/jb.84.1.137-146.1962. View