Stimulation of Transport into Escherichia Coli Membrane Vesicles by Internally Generated Reduced Nictotinamide Adenine Dinucleotide

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Nov 1

PMID 4156360

Citations 7

Authors

M Futai

Affiliations

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Abstract

Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about 60 to 80% of that stimulated by d-lactate. This transport was inhibited by cyanide and anaerobiosis. Ferricyanide, a nonpermeable electron acceptor, inhibited transport stimulated by external NADH, but not that stimulated by internally generated NADH.

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