Mutants of Nonproducer Cell Lines Transformed by Murine Sarcoma Virus. II. Relationship of Tumorigenicity to Presence of Viral Markers and Rescuable Sarcoma Genome

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1973 Aug 1

PMID 4124209

Citations 5

Authors

M Hatanaka

R Klein

C W Lomg

R Gilden

Affiliations

Soon will be listed here.

Abstract

Tumorigenic and nontumorigenic mutants induced by a single 5'-bromodeoxyuridine (BrdU) treatment of a nonproducer (NP) tumorigenic cell line were isolated and characterized. Among the cloned derivatives were examples of virus-free and sarcoma virus-producing cell lines. Oncogenicity did not correlate with production of virus or ease of rescue of the sarcoma genome. All lines, including nononcogenic derivatives, retained the sarcoma genome. Phenotypic reversion of some cell mutants was observed after in vivo inoculation or long term in vitro cultivation. The M-50T cell line, obtained from a tumor induced by M-50 cells, had a sarcoma genome rescuable by direct superinfection; this was only achieved with parental M-50 cells by a cell fusion rescue technique. The M-43-2T cell, obtained from a single small static tumor induced by otherwise nononcogenic M-43-2 cells, shed sarcoma virus and became tumorigenic. M-58-4-48 became tumorigenic after passage 48 of the M-58-4 line, which was originally nontumorigenic. These observations of phenotypic reversion demonstrate that the presence of the sarcoma gene in cells is an essential but not sufficient condition of tumorigenesis.

Citing Articles

Selection of revertants of Kirsten sarcoma virus transformed nonproducer BALB-3T3 cells.

Ozanne B, Vogel A J Virol. 1974; 14(2):239-48.

PMID: 4367900 PMC: 355509. DOI: 10.1128/JVI.14.2.239-248.1974.

Mutants of nonproducer cell lines transformed by murine sarcoma virus. 3. Detection and characterization of RNA specific for helper and sarcoma viruses.

Tsuchida N, Shih M, Gilden R, Hatanaka M J Exp Med. 1974; 140(1):218-24.

PMID: 4365514 PMC: 2139702. DOI: 10.1084/jem.140.1.218.

Sarcoma-virus-related RNA sequences in normal rat cells.

Tsuchida N, Gilden R, Hatanaka M Proc Natl Acad Sci U S A. 1974; 71(11):4503-7.

PMID: 4140505 PMC: 433915. DOI: 10.1073/pnas.71.11.4503.

Sarcoma and helper-specific RNA tumor virus subunits in transformed nonproducer mouse cells activated to produce virus by treatment with bromodeoxyuridine.

Tsuchida N, SHIH M, Gilden R, Hatanaka M J Virol. 1974; 14(5):1262-7.

PMID: 4139290 PMC: 355643. DOI: 10.1128/JVI.14.5.1262-1267.1974.

Tumorigenicity of Indian muntjac diploid cells by the proviral integration of sarcoma gene of a mouse retrovirus.

Hatanaka M, Klein R J Exp Med. 1979; 150(5):1195-201.

PMID: 501287 PMC: 2185692. DOI: 10.1084/jem.150.5.1195.

References

HUEBNER R, Hartley J, Rowe W, LANE W, CAPPS W . Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966; 56(4):1164-9. PMC: 220030. DOI: 10.1073/pnas.56.4.1164. View

Hatanaka M, Klein R, Toni R, Walker J, Gilden R . Mutants of nonproducer cell lines transformed by murine sarcoma viruses. I. Induction, isolation, particle production, and tumorigenicity. J Exp Med. 1973; 138(2):356-63. PMC: 2139394. DOI: 10.1084/jem.138.2.356. View

Hartley J, Rowe W, CAPPS W, HUEBNER R . Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969; 3(2):126-32. PMC: 375741. DOI: 10.1128/JVI.3.2.126-132.1969. View

Harris H, MILLER O, Klein G, Worst P, Tachibana T . Suppression of malignancy by cell fusion. Nature. 1969; 223(5204):363-8. DOI: 10.1038/223363a0. View

Rabinowitz Z, Sachs L . Control of the reversion of properties in transformed cells. Nature. 1970; 225(5228):136-9. DOI: 10.1038/225136a0. View

Hartley J, Rowe W, HUEBNER R . Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970; 5(2):221-5. PMC: 375990. DOI: 10.1128/JVI.5.2.221-225.1970. View

Bassin R, Tuttle N, Fischinger P . Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int J Cancer. 1970; 6(1):95-107. DOI: 10.1002/ijc.2910060114. View

Silagi S, Bruce S . Suppression of malignancy and differentiation in melanotic melanoma cells. Proc Natl Acad Sci U S A. 1970; 66(1):72-8. PMC: 286089. DOI: 10.1073/pnas.66.1.72. View

Pollack R, Wolman S, Vogel A . Reversion of virus-transformed cell lines: hyperploidy accompanies retention of viral genes. Nature. 1970; 228(5275):938 passim. DOI: 10.1038/228938a0. View

10.

Rowe W, PUGH W, Hartley J . Plaque assay techniques for murine leukemia viruses. Virology. 1970; 42(4):1136-9. DOI: 10.1016/0042-6822(70)90362-4. View

11.

Hitotsumachi S, Rabinowitz Z, Sachs L . Ciromosomal control of reversion in transformed cells. Nature. 1971; 231(5304):511-4. DOI: 10.1038/231511a0. View

12.

Bassin R, Phillips L, Kramer M, Haapala D, Peebles P, Nomura S . Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus. Proc Natl Acad Sci U S A. 1971; 68(7):1520-4. PMC: 389231. DOI: 10.1073/pnas.68.7.1520. View

13.

Aaronson S . Chemical induction of focus-forming virus from nonproducer cells transformed by murine sarcoma virus. Proc Natl Acad Sci U S A. 1971; 68(12):3069-72. PMC: 389592. DOI: 10.1073/pnas.68.12.3069. View

14.

Kellogg G, HUEBNER R, Long C, Gilden R . Rapid rescue of the defective M-MSV genome by the use of cell fusion. Virology. 1971; 46(3):965-8. DOI: 10.1016/0042-6822(71)90098-5. View

15.

Fischinger P, Schaefer W, Seifert E . Detection of some murine leukemia virus antigens in virus particles derived from 3T3 cells transformed only by murine sarcoma virus. Virology. 1972; 47(1):229-35. DOI: 10.1016/0042-6822(72)90254-1. View

16.

Kelloff G, Hatanaka M, Gilden R . Assay of C-type virus infectivity by measurement of RNA-dependent DNA polymerase activity. Virology. 1972; 48(1):266-9. DOI: 10.1016/0042-6822(72)90135-3. View

17.

Nomura S, Fischinger P, MATTERN C, Peebles P, Bassin R, Friedman G . Revertants of mouse cells transformed by murine sarcoma virus. I. Characterization of flat and transformed sublines without a rescuable murine sarcoma virus. Virology. 1972; 50(1):51-64. DOI: 10.1016/0042-6822(72)90345-5. View

18.

Stephenson J, Scolnick E, Aaronson S . Genetic stability of the sarcoma viruses in murine and avian sarcoma virus-transformed nonproducer cells. Int J Cancer. 1972; 9(3):577-83. DOI: 10.1002/ijc.2910090314. View

19.

Rabinowitz Z, Sachs L . Reversion of properties in cells transformed by polyoma virus. Nature. 1968; 220(5173):1203-6. DOI: 10.1038/2201203a0. View