Dissociation by Chelating Agents and Substructure of the Thermophilic Bacteriophage TP84

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1971 May 1

PMID 4104254

Citations 7

Authors

A BASSEL

M Shaw

L L Campbell

Affiliations

Soon will be listed here.

Abstract

The thermophilic bacteriophage TP84 is dissociated into its head, tail, and released deoxyribonucleic acid (DNA) by chelating agents such as ethylenediaminetetraacetic acid (EDTA) and phosphate. The phage is more sensitive to EDTA than to phosphate, and dialysis against either agent causes more effective dissociation than standing in their presence. The tail possesses a knobbed structure which is inserted into the head of the intact phage and to which the DNA appears to be attached. The method of dissociating TP84 described in this paper provides a source of undamaged structural components and intact strands of DNA for subsequent investigations. A possible mechanism of chelate inactivation is discussed.

Citing Articles

Potential Applications of Thermophilic Bacteriophages in One Health.

Liu H, Kheirvari M, Tumban E Int J Mol Sci. 2023; 24(9).

PMID: 37175929 PMC: 10179064. DOI: 10.3390/ijms24098222.

A novel thermostable TP-84 capsule depolymerase: a method for rapid polyethyleneimine processing of a bacteriophage-expressed proteins.

Lubkowska B, Czajkowska E, Stodolna A, Sroczynski M, Zylicz-Stachula A, Sobolewski I Microb Cell Fact. 2023; 22(1):80.

PMID: 37098567 PMC: 10131341. DOI: 10.1186/s12934-023-02086-2.

Practical Method for Isolation of Phage Deletion Mutants.

Gutierrez D, Fernandez L, Rodriguez A, Garcia P Methods Protoc. 2019; 1(1).

PMID: 31164553 PMC: 6526443. DOI: 10.3390/mps1010006.

Sequence, genome organization, annotation and proteomics of the thermophilic, 47.7-kb Geobacillus stearothermophilus bacteriophage TP-84 and its classification in the new Tp84virus genus.

Skowron P, Kropinski A, Zebrowska J, Janus L, Szemiako K, Czajkowska E PLoS One. 2018; 13(4):e0195449.

PMID: 29624616 PMC: 5889276. DOI: 10.1371/journal.pone.0195449.

Disruptive action of potassium phosphotungstate on phiT, the temperate bacteriophage of Bacillus megaterium 899a.

Hendry G, Fitz-James P J Virol. 1974; 13(2):529-31.

PMID: 4129795 PMC: 355324. DOI: 10.1128/JVI.13.2.529-531.1974.

References

Yamamoto N, Fraser D, Mahler H . Chelating agent shock of bacteriophage T5. J Virol. 1968; 2(9):944-50. PMC: 375716. DOI: 10.1128/JVI.2.9.944-950.1968. View

Shafia F, Thompson T . Isolation and preliminary characterization of bacteriophage phi-mu-4. J Bacteriol. 1964; 87(5):999-1002. PMC: 277137. DOI: 10.1128/jb.87.5.999-1002.1964. View

Van Vunakis H, HERRIOTT R . Inactivation of the T-even coliphages by pyrophosphate. J Bacteriol. 1962; 83:590-6. PMC: 279316. DOI: 10.1128/jb.83.3.590-596.1962. View

Luftig R, Haselkorn R . Studies on the structure of blue-green algae virus LPP-1. Virology. 1968; 34(4):664-74. DOI: 10.1016/0042-6822(68)90087-1. View

Luftig R, Haselkorn R . Morphology of a virus of blue-green algae and properties of its deoxyribonucleic acid. J Virol. 1967; 1(2):344-61. PMC: 375233. DOI: 10.1128/JVI.1.2.344-361.1967. View

Egbert L, MITCHELL H . Characteristics of Tphi3, a bacteriophage for Bacillus stearothermophilus. J Virol. 1967; 1(3):610-6. PMC: 375290. DOI: 10.1128/JVI.1.3.610-616.1967. View

White R, GEORGI C, MILITZER W . Heat studies on a thermophilic bacteriophage. Proc Soc Exp Biol Med. 1954; 85(1):137-9. DOI: 10.3181/00379727-85-20809. View

Cummings D, CHAPMAN V, DELONG S . Disruption of T-even bacteriophages by dimethyl sulfoxide. J Virol. 1968; 2(6):610-20. PMC: 375655. DOI: 10.1128/JVI.2.6.610-620.1968. View

Saunders G, Campbell L . Characterization of a thermophilic bacteriophage for Bacillus stearothermophilus. J Bacteriol. 1966; 91(1):340-8. PMC: 315953. DOI: 10.1128/jb.91.1.340-348.1966. View

10.

Lark K, Adams M . The stability of phages as a function of the ionic environment. Cold Spring Harb Symp Quant Biol. 1953; 18:171-83. DOI: 10.1101/sqb.1953.018.01.028. View

11.

Saunders G, Campbell L . Properties of the deoxyribonucleic acid of the thermophilic bacteriophage TP-84. Biochemistry. 1965; 4(12):2836-44. DOI: 10.1021/bi00888a039. View

12.

VILLAREJO M, Hua S, EVANS Jr E . Protein components of bacteriophages lambda and lambda virulent. J Virol. 1967; 1(5):928-34. PMC: 375371. DOI: 10.1128/JVI.1.5.928-934.1967. View

13.

White R, GEORGI C, MILITZER W . Characteristics of a thermophilic bacteriophage. Proc Soc Exp Biol Med. 1955; 88(3):373-7. DOI: 10.3181/00379727-88-21592. View

14.

BRADLEY D . Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967; 31(4):230-314. PMC: 408286. DOI: 10.1128/br.31.4.230-314.1967. View

15.

Ames B, DUBIN D . The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960; 235:769-75. View

16.

Shafia F, Thompson T . CALCIUM ION REQUIREMENT FOR PROLIFERATION OF BACTERIOPHAGE PHI MU-4. J Bacteriol. 1964; 88:293-6. PMC: 277298. DOI: 10.1128/jb.88.2.293-296.1964. View