Production and Purification of the Thermophilic Bacteriophage TP-84

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1975 Feb 1

PMID 234714

Citations 11

Authors

I Epstein

L L Campbell

Affiliations

Soon will be listed here.

Abstract

A new procedure for production and purification of the thermophilic bacteriophage TP-84 in high yields is described. Cultures of Bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a pH of 6.5, were infected with the phage in a 100-liter fermentor. Addition of magnesium chloride (0.01 M) and a temperature of 58-C were essential for maximal phage production. Phage (5 times 1011 infective particles/ml) was precipitated with polyethylene glycol (molecular weight 6,000) in the presence of sodium chloride and was further purified by cesium chloride density centrifugation.

Citing Articles

New-Generation Antibacterial Agent-Cellulose-Binding Thermostable TP84_Endolysin.

Ponikowska M, Zebrowska J, Skowron P Int J Mol Sci. 2024; 25(23).

PMID: 39684821 PMC: 11642820. DOI: 10.3390/ijms252313111.

Recombinant TP-84 Bacteriophage Glycosylase-Depolymerase Confers Activity against Thermostable via Capsule Degradation.

Lubkowska B, Sobolewski I, Adamowicz K, Zylicz-Stachula A, Skowron P Int J Mol Sci. 2024; 25(2).

PMID: 38255796 PMC: 10815759. DOI: 10.3390/ijms25020722.

A Method for Rapid Polyethyleneimine-Based Purification of Bacteriophage-Expressed Proteins from Diluted Crude Lysates, Exemplified by Thermostable TP-84 Depolymerase.

Lubkowska B, Czajkowska E, Sobolewski I, Krawczun N, Zylicz-Stachula A, Skowron P Microorganisms. 2023; 11(9).

PMID: 37764184 PMC: 10535936. DOI: 10.3390/microorganisms11092340.

A novel thermostable TP-84 capsule depolymerase: a method for rapid polyethyleneimine processing of a bacteriophage-expressed proteins.

Lubkowska B, Czajkowska E, Stodolna A, Sroczynski M, Zylicz-Stachula A, Sobolewski I Microb Cell Fact. 2023; 22(1):80.

PMID: 37098567 PMC: 10131341. DOI: 10.1186/s12934-023-02086-2.

A Method for Isolation Bacteriophage Particles-Free Genomic DNA, Exemplified by TP-84, Infecting Thermophilic .

Sobolewski I, Adamowicz K, Struck A, Zylicz-Stachula A, Skowron P Microorganisms. 2022; 10(9).

PMID: 36144384 PMC: 9502220. DOI: 10.3390/microorganisms10091782.

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