Natural Product-Derived Senotherapeutics: Extraction and Biological Evaluation Techniques

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2025 Mar 14

PMID 40082365

Authors

Harris Pratsinis

Eleni Mavrogonatou

Sevasti-Kiriaki Zervou

Theodoros Triantis

Anastasia Hiskia

Dimitris Kletsas

Affiliations

Soon will be listed here.

Abstract

Selective targeting of senescent cells has been thus far considered a widespread preventive strategy, as well as a main or adjuvant therapy for age-associated diseases, fueling the research on the discovery of senotherapeutics (i.e., senolytic or senomorphic compounds). Given that until now no single senotherapeutic has been reported to exert a universal anti-senescence action due to the cell- /tissue-, and context-dependent specificity of such compounds, seeking novel selective senotherapeutics remains of great importance. In this chapter, a research strategy that could be followed to screen natural product collections for putative senotherapeutics with enhanced specificity and reduced toxicity is presented, from the extraction of the source material and the isolation and chemical characterization of the compounds of interest to their biological evaluation in vitro and in vivo.

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