Isolation and Characterization of Endothelial-Colony Forming Cells (ECFC): Studying Endothelial Senescence for Translational Studies and for Personalized Medicine

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2025 Mar 14

PMID 40082361

Authors

Koralia Paschalaki

Charis Pericleous

Affiliations

Soon will be listed here.

Abstract

Endothelium regulates vascular homeostasis maintaining a healthy cardiovascular system but also plays a key role in tumor development, progression, and metastasis. Endothelial senescence can be driven by aging, DNA damage, oxidative stress, oncogenes and chemotherapy, and contributes to vascular dysfunction. Endothelial colony-forming cells (ECFC) are endothelial-committed progenitors with clonogenic potential, de novo angiogenic capacity and endothelial regenerative abilities. Studying ECFC senescence provides a novel approach to investigate the molecular mechanisms of endothelial dysfunction and response to treatment, in a noninvasive and personalized manner.

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