Hemogregarines in Bufonid Anurans from the Brazilian Amazon, with Description a New Species of (Apicomplexa: Lankesterellidae)

Overview

Journal Int J Parasitol Parasites Wildl

Date 2025 Mar 4

PMID 40034141

Authors

Tassio Alves-Coelho

Darlison Chagas-de-Souza

Carolina Romeiro Fernandes Chagas

German Alfredo Gutierrez-Liberato

Livia Perles

Amir Alabi

Marcos Rogerio Andre

Lucio Andre Viana

Affiliations

Soon will be listed here.

Abstract

Here we describe, in an unprecedented way for the Brazilian Amazon, one Apicomplexa species infecting an anuran in Pará, Brazil, using an integrative approach that includes taxonomy, providing morphology, morphometrics, and molecular data. Samples were collected between February 2021 and February 2022 in the community of Curupira, municipality of Santarém, and in the industrial area of Marabá, both in Pará state. Specimens of and were captured by hand during the active search, and blood samples were collected by cardiac puncture. One blood aliquot was used to prepare blood smears for microscopical analysis and another aliquot for further molecular analyses. Of the five caught, one (20%) presented parasites morphologically compatible with the parasites belonging to the genus , while of the three caught, one (33.3%) presented parasites morphologically compatible with the parasites belong to the genera and . Based on molecular data of rDNA sequences, the sequence obtained clustered with other parasite species recovered from Brazilian amphibians. The sp. sequence was placed in a sister clade of species described in birds and close to , described in amphibians. All attempts to amplify parasite DNA were unsuccessful. We described n. sp. and reported infection by sp. and sp. in bufonids anurans from the Brazilian Amazon. This study increases knowledge of Brazilian anuran hemoparasites and confirms the importance of using an integrative approach for the taxonomy of these parasite groups.

References

Netherlands E, Cook C, du Preez L, Vanhove M, Brendonck L, Smit N . Monophyly of the species of Hepatozoon (Adeleorina: Hepatozoidae) parasitizing (African) anurans, with the description of three new species from hyperoliid frogs in South Africa. Parasitology. 2017; 145(8):1039-1050. PMC: 6084435. DOI: 10.1017/S003118201700213X. View

Netherlands E, Cook C, Smit N, du Preez L . Redescription and molecular diagnosis of Hepatozoon theileri (Laveran, 1905) (Apicomplexa: Adeleorina: Hepatozoidae), infecting Amietia quecketti (Anura: Pyxicephalidae). Folia Parasitol (Praha). 2014; 61(4):293-300. View

Barta J, Martin D, Carreno R, Siddall M, Hozza M, Powles M . Molecular phylogeny of the other tissue coccidia: Lankesterella and Caryospora. J Parasitol. 2001; 87(1):121-7. DOI: 10.1645/0022-3395(2001)087[0121:MPOTOT]2.0.CO;2. View

Smith T . The genus Hepatozoon (Apicomplexa: Adeleina). J Parasitol. 1996; 82(4):565-85. View

Netherlands E, Svitin R, Cook C, Smit N, Brendonck L, Vanhove M . Neofoleyellides boerewors n. gen. n. sp. (Nematoda: Onchocercidae) parasitising common toads and mosquito vectors: morphology, life history, experimental transmission and host-vector interaction in situ. Int J Parasitol. 2020; 50(3):177-194. DOI: 10.1016/j.ijpara.2019.11.009. View

Ungari L, Santos A, ODwyer L, da Silva M, Rodrigues Santos T, Rodrigues da Cunha M . Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil. Parasitol Res. 2018; 117(12):3857-3865. DOI: 10.1007/s00436-018-6092-3. View

Megia-Palma R, Martinez J, Paranjpe D, DAmico V, Aguilar R, Palacios M . Phylogenetic analyses reveal that Schellackia parasites (Apicomplexa) detected in American lizards are closely related to the genus Lankesterella: is the range of Schellackia restricted to the Old World?. Parasit Vectors. 2017; 10(1):470. PMC: 5633878. DOI: 10.1186/s13071-017-2405-0. View

Ronquist F, Huelsenbeck J . MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003; 19(12):1572-4. DOI: 10.1093/bioinformatics/btg180. View

Viana L, Soares P, Silva J, Paiva F, Coutinho M . Anurans as paratenic hosts in the transmission of Hepatozoon caimani to caimans Caiman yacare and Caiman latirostris. Parasitol Res. 2011; 110(2):883-6. DOI: 10.1007/s00436-011-2570-6. View

10.

Leal D, Odwyer L, Ribeiro V, Silva R, Ferreira V, Rodrigues R . Hemoparasites of the genus Trypanosoma (Kinetoplastida: Trypanosomatidae) and hemogregarines in Anurans of the São Paulo and Mato Grosso do Sul States - Brazil. An Acad Bras Cienc. 2009; 81(2):199-206. DOI: 10.1590/s0001-37652009000200006. View

11.

Ungari L, Netherlands E, Santos A, de Alcantara E, Emmerich E, Silva R . A new species, Dactylosoma piperis n. sp. (Apicomplexa, Dactylosomatidae), from the pepper frog Leptodactylus labyrinthicus (Anura, Leptodactylidae) from Mato Grosso State, Brazil. Parasite. 2020; 27:73. PMC: 7746082. DOI: 10.1051/parasite/2020070. View

12.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

13.

Pinho S, Rodriguez-Malaga S, Lozano-Osorio R, Correa F, Silva I, Santos-Costa M . Effects of the habitat on anuran blood parasites in the Eastern Brazilian Amazon. An Acad Bras Cienc. 2021; 93(suppl 4):e20201703. DOI: 10.1590/0001-3765202120201703. View

14.

Mendoza-Roldan J, Ribeiro S, Castilho-Onofrio V, Grazziotin F, Rocha B, Ferreto-Fiorillo B . Mites and ticks of reptiles and amphibians in Brazil. Acta Trop. 2020; 208:105515. DOI: 10.1016/j.actatropica.2020.105515. View

15.

Bernotiene R, Palinauskas V, Iezhova T, Murauskaite D, Valkiunas G . Avian haemosporidian parasites (Haemosporida): A comparative analysis of different polymerase chain reaction assays in detection of mixed infections. Exp Parasitol. 2016; 163:31-7. DOI: 10.1016/j.exppara.2016.01.009. View

16.

Kvicerova J, Pakandl M, Hypsa V . Phylogenetic relationships among Eimeria spp. (Apicomplexa, Eimeriidae) infecting rabbits: evolutionary significance of biological and morphological features. Parasitology. 2008; 135(4):443-52. DOI: 10.1017/S0031182007004106. View

17.

Picelli A, Silva M, Correa J, Paiva G, Paula F, Hernandez-Ruz E . Hepatozoon Miller, 1908 parasites in the Colubridae snakes Clelia clelia (Daudin, 1803) and Drymarchon corais (Boie, 1827) from the Eastern Amazonia. An Acad Bras Cienc. 2023; 95(suppl 1):e20220115. DOI: 10.1590/0001-3765202320220115. View

18.

Oliveiraa E, Coelhoc T, De Souzac D, Correaa L, Kawashita-Ribeiroc R . New anuran amphibian host for Hemolivia sp. (Adeleorina: Karyolysidae) in the Neotropical region. Ann Parasitol. 2021; 66(4):581-585. DOI: 10.17420/ap6604.301. View

19.

Keckeisen C, Sujanova A, Himmel T, Matt J, Nedorost N, Chagas C . and Parasites (Eimeriidae, Apicomplexa) of Passeriform Birds in Europe: Infection Rates, Phylogeny, and Pathogenicity. Pathogens. 2024; 13(4). PMC: 11053544. DOI: 10.3390/pathogens13040337. View

20.

Demoner L, da Silva M, Magro N, ODwyer L . Hepatozoon milleri sp. nov. (Adeleorina: Hepatozoidae) in Akodon montensis (Rodentia: Cricetidae: Sigmodontinae) from southeastern Brazil. Parasitology. 2018; 146(5):662-669. DOI: 10.1017/S0031182018001956. View