Enzymatic, Cellular Breakdown and Lysis in Treatment of Beauveria Brongniartii on Spodoptera Litura (Fabricius, 1775)

Overview

Journal Sci Rep

Specialty Science

Date 2025 Feb 28

PMID 40021686

Authors

Perumal Vivekanandhan

Kannan Swathy

Pittarate Sarayut

Krutmuang Patcharin

Affiliations

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Abstract

The current study aimed to isolate Beauveria brongniartii conidia from forest soils, identify the fungus, and evaluate its effectiveness on the eggs, larvae, pupae, and adults of Spodoptera litura. Insect mortality rates were recorded every 3, 6, 9, and 12 days. The identification of entomopathogenic fungi was carried out using molecular techniques, including PCR, DNA sequencing, and molecular markers, to detect species-specific 18 S rDNA genetic sequences, all performed under aseptic conditions. The results indicated that higher conidia concentrations (2.7 × 10 conidia/mL) exhibited greater virulence, with eggs showing a mortality rate of 98.66%, followed by larvae 96%, adults 90.66%, and pupae 77.33% after 12 days. Probit analysis revealed minimal LC and LC values: eggs (5.5 × 10; 1.0 × 10 spores/mL), larvae (8.2 × 10; 1.2 × 10 spores/mL), pupae (9.6 × 10; 7.3 × 10 spores/mL), and adults (1.0 × 10; 2.0 × 10 spores/mL). The total hemocyte counts and detailed observational results revealed that B. brongniartii induces cellular breakdown and cell lysis in S. litura larvae by producing enzymes that degrade the cuticle and cell membranes. Earthworm bioindicator studies showed minimal effects from B. brongniartii conidia compared to controls, while chemical treatments resulted in 96% mortality at 100 ppm. Histopathological examinations revealed no significant differences in gut tissue between earthworms treated with fungal conidia and those in the control group, unlike the substantial damage caused by chemical treatments. Biochemical analysis revealed significant alterations in enzyme activity, including reduced levels of phosphatase and catalase, as well as increased levels of lipid peroxides and superoxide dismutase. This study highlights the effectiveness of B. brongniartii in controlling S. litura, demonstrating its potential as a viable biocontrol agent and promoting eco-friendly alternatives to chemical pesticides, with no risk to non-target species or the environment.

References

Islam S, Chowdhury M, Mim M, Momtaz M, Islam T . Biocontrol potential of native isolates of Beauveria bassiana against cotton leafworm Spodoptera litura (Fabricius). Sci Rep. 2023; 13(1):8331. PMC: 10205733. DOI: 10.1038/s41598-023-35415-x. View

Shaukat R, Freed S, Ahmed R, Raza M, Naeem A . Virulence and transgenerational effects of Metarhizium anisopliae on Oxycarenus hyalinipennis. Pest Manag Sci. 2023; 79(10):3843-3851. DOI: 10.1002/ps.7568. View

Goble T, Costet L, Robene I, Nibouche S, Rutherford R, Conlong D . Beauveria brongniartii on white grubs attacking sugarcane in South Africa. J Invertebr Pathol. 2012; 111(3):225-36. DOI: 10.1016/j.jip.2012.08.010. View

Perumal V, Kannan S, Alford L, Pittarate S, Krutmuang P . Study on the virulence of Metarhizium anisopliae against Spodoptera frugiperda (J. E. Smith, 1797). J Basic Microbiol. 2024; 64(5):e2300599. DOI: 10.1002/jobm.202300599. View

Staczek S, Zdybicka-Barabas A, Pleszczynska M, Wiater A, Cytrynska M . Aspergillus niger α-1,3-glucan acts as a virulence factor by inhibiting the insect phenoloxidase system. J Invertebr Pathol. 2020; 171:107341. DOI: 10.1016/j.jip.2020.107341. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Mantzoukas S, Lagogiannis I, Kitsiou F, Eliopoulos P . Entomopathogenic Action of Wild Fungal Strains against Stored Product Beetle Pests. Insects. 2023; 14(1). PMC: 9862338. DOI: 10.3390/insects14010091. View

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

Vivekanandhan P, Swathy K, Kalaimurugan D, Ramachandran M, Yuvaraj A, Kumar A . Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and non-targeting organisms. PLoS One. 2020; 15(5):e0232172. PMC: 7197772. DOI: 10.1371/journal.pone.0232172. View

10.

Shah F, Razaq M, Ali Q, Shad S, Aslam M, Hardy I . Field evaluation of synthetic and neem-derived alternative insecticides in developing action thresholds against cauliflower pests. Sci Rep. 2019; 9(1):7684. PMC: 6531477. DOI: 10.1038/s41598-019-44080-y. View

11.

Wang C, Yue X, Lu X, Liu B . The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix. Fish Shellfish Immunol. 2012; 34(1):91-9. DOI: 10.1016/j.fsi.2012.10.013. View

12.

Vivekanandhan P, Kavitha T, Karthi S, Senthil-Nathan S, Shivakumar M . Toxicity of Beauveria bassiana-28 Mycelial Extracts on Larvae of Culex quinquefasciatus Mosquito (Diptera: Culicidae). Int J Environ Res Public Health. 2018; 15(3). PMC: 5876985. DOI: 10.3390/ijerph15030440. View

13.

Marklund S, Marklund G . Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3):469-74. DOI: 10.1111/j.1432-1033.1974.tb03714.x. View

14.

Vivekanandhan P, Swathy K, Alford L, Pittarate S, Subala S, Mekchay S . Toxicity of Metarhizium flavoviride conidia virulence against Spodoptera litura (Lepidoptera: Noctuidae) and its impact on physiological and biochemical activities. Sci Rep. 2022; 12(1):16775. PMC: 9537412. DOI: 10.1038/s41598-022-20426-x. View

15.

Diaz-Albiter H, Mitford R, Genta F, SantAnna M, Dillon R . Reactive oxygen species scavenging by catalase is important for female Lutzomyia longipalpis fecundity and mortality. PLoS One. 2011; 6(3):e17486. PMC: 3052318. DOI: 10.1371/journal.pone.0017486. View

16.

Perumal V, Kannan S, Alford L, Pittarate S, Mekchay S, Reddy G . Biocontrol effect of entomopathogenic fungi Metarhizium anisopliae ethyl acetate-derived chemical molecules: An eco-friendly anti-malarial drug and insecticide. Arch Insect Biochem Physiol. 2023; 114(2):1-19. DOI: 10.1002/arch.22037. View

17.

Vivekanandhan P, Alahmadi T, Ansari M . Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae). J Basic Microbiol. 2024; 64(5):e2300744. DOI: 10.1002/jobm.202300744. View

18.

Vivekanandhan P, Swathy K, Sarayut P, Patcharin K . Biology, classification, and entomopathogen-based management and their mode of action on (Meyrick) in Asia. Front Microbiol. 2024; 15:1429690. PMC: 11335496. DOI: 10.3389/fmicb.2024.1429690. View

19.

Asakura T . Hemoglobin porphyrin modification. Methods Enzymol. 1978; 52:447-55. DOI: 10.1016/s0076-6879(78)52049-1. View

20.

Vivekanandhan P, Bedini S, Shivakumar M . Isolation and identification of entomopathogenic fungus from Eastern Ghats of South Indian forest soil and their efficacy as biopesticide for mosquito control. Parasitol Int. 2020; 76:102099. DOI: 10.1016/j.parint.2020.102099. View