Alters Gut Microbiota and Metabolites Composition to Improve High Starch Metabolism in

Overview

Journal Animals (Basel)

Date 2025 Feb 26

PMID 40003065

Authors

Linjie Qian

Siyue Lu

Wenqiang Jiang

Qiaoqiao Mu

Yan Lin

Zhengyan Gu

Yeyang Wu

Xianping Ge

Linghong Miao

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to explore the effects of adding (LAB) to a high-starch diet on glucose and lipid metabolism, gut microbiota, and the composition of metabolites in . This experiment was equipped with three isonitrogenous and isoenergetic feeds as control group (LW), high starch group (HW), and high starch with LAB group (HP). A total of 180 experimental fish (13.5 ± 0.5 g) were randomly divided into three treatments, and three floating cages (1 m × 1 m × 1 m) were set up for each treatment. A total of 20 fish per net were kept in an outdoor pond for 8 weeks. The results showed that both the HW and HP groups had an altered structure and a reduced diversity of gut microbiota. LAB increased the abundance of and the ratio of Firmicutes/Bacteroidota and decreased PC (16:1/20:5) and taurochenodeoxycholic acid levels. LAB promoted the expression of genes related to the intestinal bile acid cycle (, , , and ) and inhibited the expression of and ( < 0.05). LAB reduced the expression of genes related to transported cholesterol ( and ) ( < 0.05) in the liver. In conclusion, LAB addition could regulate the gut microbiota disorders caused by high starch levels, promote cholesterol metabolism, produce bile acids, and reduce lipid deposition.

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